Pigsties near dwellings as a potential risk factor for the prevalence of Japanese encephalitis virus in adult in Shanxi,China

作     者：Xiaojie Ren Shihong Fu Peifang Dai Huanyu Wang Yuanyuan Li Xiaolong Li Wenwen Lei Xiaoyan Gao Ying He Zhi Lv Jingxia Cheng Guiqin Wang Guodong Liang 

作者机构：Department of Immunology and MicrobiologyShanxi Medical UniversityTaiyuan 030001China State Key Laboratory of Infectious Disease Prevention and ControlNational Institute for Viral Disease Control and PreventionChinese Center for Disease Control and PreventionBeijing 102206China Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseasesHangzhou 310058China Shanxi Center for Disease Control and PreventionTaiyuan 030001China 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2017年第6卷第1期

页      面：900-910页

核心收录：

学科分类：0202[经济学-应用经济学] 02[经济学] 020205[经济学-产业经济学] 

基　　金：This work was supported by grants from National Natural Science Foundation of China(81290342 and 81501757) Development Grant of State Key Laboratory of Infectious Disease Prevention and Control(2014SKLID103) 

主　　题：Adult Japanese encephalitis Epidemic disease Mosquito-borne arbovirus Japanese encephalitis virus 

摘      要：Background:The increasing trend of adult cases of Japanese encephalitis(JE)in China,particularly in northern China,has become an important public health *** conducted an epidemiological investigation in the south of Shanxi Province to examine the relationships between mosquitoes,Japanese encephalitis virus(JEV),and adult JE ***:Mosquito specimens were collected from the courtyards of farmers’households and pig farms in Shanxi *** were pooled,homogenized,and *** transcription-polymerase chain reaction(RT-PCR)was used to detect mosquito-borne arbovirus genes in *** positive for these genes were inoculated into the baby hamster kidney cell line(BHK-21)to isolate *** infection rate was calculated and phylogenetic analyses were ***:A total of 7943 mosquitoes belonging to six species in four genera were collected;Culex tritaeniorhynchus accounted for 73.08%(5805/7943),*** pallens for 24.75%(1966/7943),and the remaining 3%(104/7943)consisted of Anopheles sinensis,Aedes vexans,***,and Armigeres *** pools were positive for JEV based on RT-PCR using JEV pre-membrane gene nested *** analyses showed that all JEVs belonged to genotype I;two pools were positive using Getah Virus(GETV)gene *** addition,one JEV strain(SXYC1523)was isolated from *** pallens *** results indicate that the minimum infection rate of JEV in mosquito specimens collected from the courtyards of farmers’households with pigsties was 7.39/1000;the rate for pig farms was 2.68/1000;and the rate for farmers’courtyards without pigsties was ***:The high-prevalence regions of adult JE investigated in this study are still the natural epidemic focus of *** pigsties near dwellings is a potential risk factor contributing to the prevalence of adult *** prevent the occurrence of local adult JE cases,a recommendation was raised that,besides con