An automated multiwell plate reading flim microscope for live cell autofluorescence lifetime assays

作     者：Douglas J.Kelly Sean C.Warren Sunil Kumar Joao L.Lagarto Benjamin T.Dyer Anca Margineanu Eric W.-F.Lam Chris Dunsby Paul M.W.French 

作者机构：Photonics GroupDepartment of PhysicsImperial College London South Kensington CampusLondonSW72AZUK Institute of Chemical BiologyDepartment of Chemistry Imperial College LondonSouth Kensington Campus LondonSWT 2AZUK National Heart&Lung Institute Imperial Centre for Eaperimental&Translational Medicine Du Cane RoadLondonW12 ONNUK Department of Surgery and CancerImperial Cllege London Du Cane RoadLondonW12 ONNUK Centre for HistopathologyImperial College London Du Cane RoadLondonW120NNUK 

出 版 物：《Journal of Innovative Optical Health Sciences》 (创新光学健康科学杂志（英文）)

年 卷 期：2014年第7卷第5期

页      面：99-113页

核心收录：

学科分类：0502[文学-外国语言文学] 050201[文学-英语语言文学] 05[文学] 

基　　金：funding from the UK Biotechnology and Biological Sciences Research Council(BBSRCBB/H00713X/1) the UK Engineering and PhysicalSciences Research Council(EPSRC EP/IO2770X/1) the UK Technology Strategy Board Technology Award(CHBT/007/00030,EP/C54269X,inpartnership with Astra Zeneca,GE Healthcare,GSK,Kentech Instruments Ltd) 

主　　题：High content analysis FLIM NADH metabolism cisplatin. 

摘      要：Fluorescence lifetime imaging(FLIM)is increasingly used to read out cellular autofluorescenceoriginating from the coenzyme NADH in the context of investigating cell metabolic *** here an automated multiwell plate reading FLIM microscope optimized for UV illumi-nation with the goal of extending high content fluorescence lifetime asays to readouts ofmetabolism,We demonstrate its application to automated cellular autofluorescence lifetime imaging and discuss the key practical issues associated with its *** particular,weillustrate its capability to read out the NADH-lifetime response of cells to metabolic modulators,thereby illustrating the potential of the instrument for cytotoxicity studies,assays for drugdiscovery and stratified medicine.