Study roadmap for high-throughput development of easy to use and affordable biomarkers as diagnostics for tropical diseases:a focus on malaria and schistosomiasis

作     者：Kokouvi Kassegne Ting Zhang Shen-Bo Chen Bin Xu Zhi-Sheng Dang Wang-Ping Deng Eniola Michael Abe Hai-Mo Shen Wei Hu Takele Geressu Guyo Solomon Nwaka Jun-Hu Chen Xiao-Nong Zhou 

作者机构：National Institute of Parasitic Diseases(NIPD)Chinese Centre for Disease Control and PreventionShanghai 200025People’s Republic of China WHO Collaborating Centre for Tropical DiseasesNational Centre for International Research on Tropical DiseasesKey Laboratory of Parasite and Vector Biology of the Chinese Ministry of HealthShanghai 200025People’s Republic of China Department of Microbiology and Microbial EngineeringSchool of Life ScienceFudan UniversityShanghai 200433People’s Republic of China African Network for Drugs&Diagnostics Innovation(ANDI)Addis AbabaEthiopia 

出 版 物：《Infectious Diseases of Poverty》 (贫困所致传染病（英文）)

年 卷 期：2017年第6卷第1期

页      面：1149-1156页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：On behalf of the WHA-WHO Demonstration Project,this work was granted by the United Nations Office for Project Services(UNOPS),in the context of the implementation of‘Development for Easy to Use and Affordable Biomarkers as Diagnostics for Types II and III Diseases’(Grant No.UNOPS/ANDI/G/2016/01) It has also received the support of the National Key Research and Development Program of China(Grant No.2016YFC1202000,2016YFC1202003 and 2016YFC1200500) the National Natural Science Foundation of China(Grant No.81101266) the Fourth Round of Three-Year Public Health Action Plan(2015-2017)in Shanghai(Grant No.GWIV-29 and 15GWZK0101) 

主　　题：Malaria Schistosomiasis Neglected tropical diseases Immunoproteomics Protein microarray Serological biomarker Diagnosis 

摘      要：Background:Interventions are currently being used against‘infectious diseases of poverty’,which remain highly debilitating and deadly in most endemic countries,especially malaria,schistosomiasis,echinococcosis and African sleeping ***,major limitations of current‘traditional’methods for diagnosis are neither simple nor convenient for population surveillance,and showed low sensitivity and *** to novel technologies for the development of adequate and reliable tools are expressly needed.A collaborative project between African Network for Drugs and Diagnostics Innovation and partner institutions in Africa and China aims to screen suitable serological biomarkers for diagnostic pipelines against these‘diseases of the poor’.Methods:Parasite-specific exposed versus unexposed individuals were screened and sera or urine/stools were collected through case-control studies in China and African *** genes/open reading frames were selected,then will be cloned and cell-free expressed,quantified and *** antigens/epitopes will be probed and screened with sera from exposed or unexposed individuals using a high-throughput antigen screening platform as the study *** specificity and sensitivity of highly immunoreactive biomarkers will be evaluated as well,using enzyme-linked immunosorbent assays or ***:This roadmap explicitly unfolds the integrated operating procedures with focus on malaria and schistosomiasis,for the identification of suitable biomarkers that will aid the prioritization of diagnostics for population ***,there is need to further validate any new diagnostic through comparison with standard methods in field deployable tests for each *** expectations for the future are to seek regulatory approval and promote the use of diagnostics in endemic areas.