An optimized high quality male DNA extraction from spermatophores in open thelycum shrimp species
作者机构：Laboratory of Genetic IchthyologyUniversity of GironaGironaSpain
出 版 物：《Integrative Zoology》 (整合动物学（英文版）)
年 卷 期：2017年第12卷第5期
页 面：421-427页
核心收录：
学科分类：070902[理学-地球化学] 0709[理学-地质学] 07[理学]
基 金：supported by a grant from Spanish Ministerio de Economía y Competitividad(CTM2014-54648-C2-2-R) MIR.LP benefited from predoctoral fellowship from the Universitat de Girona(BR2014)
主 题：differential spermatophore DNA extraction high quality DNA male genotyping non-invasive DNA extraction open thelycum shrimp
摘 要：The crucial step of most of the current genetic studies is the extraction of DNA of sufficient quantity and *** genomic DNA isolation methods have been described to successfully obtain male DNA from shrimp ***,all current protocols require invasive handling methods with males for DNA *** Aristeus antennatus as a model we tested a reliable non-invasive differential DNA extraction method to male DNA isolation from spermatophores attached to female *** present protocol provides high quality and quantity DNA for polymerase chain reaction amplification and male *** new approach could be useful to experimental shrimp culture to select sires with relevant genetic patterns for selective breeding *** importantly,it can be applied to identify the mating pairs and male structure in wild populations of species as ***,where males are often difficult to *** method could be also valuable for biological studies on other spermatophore-using species,such as myriapods,arachnids and insects.
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