Establishment of a New Method for Genetic Transformation of Dunaliella salina

作     者：柴晓杰 靳非 丛玉婷 岳金荣 刘艺琼 Xiaojie CHAI;Fei JIN;Yuting CONG;Jinrong YUE;Yiqiong LIU

作者机构：大连海洋大学/辽宁省水生生物学重点实验室辽宁大连116023 

出 版 物：《Agricultural Science & Technology》 (农业科学与技术（英文版）)

年 卷 期：2017年第18卷第8期

页      面：1374-1377页

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by National Natural Science Foundation of China(31472260)~~ 

主　　题：Dunaliela salina LiAc/PEG mediating method GUS gene Genetic transformation 

摘      要：The exogenous gene was integrated into Dunaliella salina successfully by using LiAc/PEG mediating method for the first time. According to the results of histochemical staining, transgenic D. salina was blue, showing that the exogenous GUS gene was successfully expressed in the cells of D. salina. Meanwhile, the effects of growth state of D. salina, plasmid concentration and temperature on its transformation efficiency were studied, and the transformation conditions were optimized. The results show that the optimum conditions for the genetic transformation of D. salina are shown as follows： D. salina was in the early logarithmic phase; plasmid DNA concentration was 600 μg/ml; temperature was 29 ℃, and transformation efficiency was up to 74.8‰ under the best conditions. According to the results of PCR amplification and PCR-Southern hybridization, the target gene had been integrated into genome and was hereditary.