Mitochondrial DNA T7719G in tRNA-Lys gene affects litter size in Small-tailed Han sheep

作     者：Xiaoyong Chen Dan Wang Hai Xiang Weitao Dun Dave O.H.Brahi Tao Yin Xingbo Zhao 

作者机构：College of Animal Science and Technology China Agricultural University Beijing 100193 China Institute of Animal Science and Veterinary of Hebei Province Baoding 071000 China 

出 版 物：《Journal of Animal Science and Biotechnology》 (畜牧与生物技术杂志（英文版）)

年 卷 期：2017年第8卷第3期

页      面：568-573页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 0905[农学-畜牧学] 0906[农学-兽医学] 09[农学] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：supported by the Project of Science and Technology of Hebei Province(15226308D) the National Key Basic Research Program of China(2014CB138500) 

主　　题：Association Haplotype Mitochondria Non synonymous mutation Reproduction Sheep 

摘      要：Background： In farm animals, mitochondrial DNA（mtDNA） effect on economic performance remains hot-topic for breeding and genetic selection. Here, 53 maternal lineages of Small-tailed Han sheep were used to investigate the association of mitochondrial DNA variations and the lambing litter ***： Sequence sweeping of the mitochondrial coding regions discovered 31 non-synonymous mutations, and the association study revealed that T7719G in mt DNA t RNA-Lys gene was associated with litter size（P 〈 0.05）,manifesting 0.29 lambs per litter between the G and T carriers. Furthermore, using the mixed linear model, we assayed the potential association of the ovine litter size and haplogroups and multiple-level mtDNA haplotypes,including general haplotypes, assembled haplotypes of electron transport chain contained sequences（H-ETC）,mitochondrial respiratory complex contained sequences（H-MRC） and mitochondrial genes（H-gene, including polypeptide-coding genes, rRNA genes and tRNA genes）. The strategy for assembled mitochondrial haplotypes was proposed for the first time in mtDNA association analyses on economic traits, although none of the significant relations could be concluded（P 〉 0.05）. In addition, the nuclear major gene BMPR1B was significantly correlated with litter size in the flock（P 〈 0.05）, however, did not interact with mtDNA T7719G mutation（P 〉 0.05）.Conclusions： Our results highlight mutations of ovine mitochondrial coding genes, suggesting T7719G in tRNA-Lys gene be a potentially useful marker for selection of sheep litter size.