Energy-coupling mechanism of the multidrug resistance transporter AcrB： Evidence for membrane potential-driving hypothesis through mutagenic analysis

作     者：Liu, Min Zhang, Xuejun C. 

作者机构：Chinese Acad Sci Inst Biophys CAS Ctr Excellence Biomacromol Natl Lab Biomacromol Beijing 100101 Peoples R China Univ Chinese Acad Sci Beijing 100049 Peoples R China 

出 版 物：《Protein & Cell》 (蛋白质与细胞（英文版）)

年 卷 期：2017年第8卷第8期

页      面：623-627页

核心收录：

学科分类：0710[理学-生物学] 12[管理学] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：The authors thank Dr. Yong Tao of the Institute of Microbiology  CAS  for providing the ΔacrB cell strain. We thank Dr. Torsten Juelich for linguistic assistance during the preparation of this manuscript. This work was supported by the National Basic Research Program (973 Program) (No. 2015CB910104 to XCZ) and National Natural Science Foundation of China (Grant No. 31470745 to XCZ) 

主　　题：突变分析 耦合机制 acrB 驱动 膜电位 转运体 证据 假说 

摘      要：Dear Editor, RND （resistance nodulation-cell division） exporters are widely expressed across the lineage of Gram-negative bacteria （Fig. S1 ）. Their functional role is to utilize the protonmotive force of the cell to drive the expulsion of cytotoxic substances from the outer leaflet of the inner membrane as well as the periplasm to extracellular space （Yamaguchi et al., 2015）.