Effects of gene silencing of CypB on gastric cancer cells
Effects of gene silencing of CypB on gastric cancer cells作者机构：Department of GastroenterologyTraining Hospital Daqing Oil Feld General Hospital Affiliated to Qiqihar Medical School
出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)
年 卷 期：2015年第8卷第4期
页 面：319-324页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：supported by Natural Science Fundation of Heilongjiang Province.China(Grant no.H2013114)
主 题：Gastric cancer CyclophilinB Gene silencing
摘 要：Objective:To determine the effect of gene silencing of cyclophilin B(CypB)on growth and proliferation of gastric cancer ***:CypB siRNA lentivirus(LV-CypB-si)and control lentivirus(LV-si-con)were *** expression in gastric cancer cell lines was detected by Western ***823 and SGC7901 cells were chosen to be infected with LV-sicon and LV-CypB-si,and stable transfectants were *** cell groups transfected with LV-CypB-siRNA,LV-siRNA-con and transfected no carrier were served as the experimental group,the implicit control group and the blank control group *** and colony formation assays were used to examine the effect of CypB on the cell growth and proliferation in *** cycle was analyzed with flow *** expression of VEGFR of BGC823-si and SGC7901-si was detected by Western ***:Gene silencing of CypB can inhibit gastric cancer cell growth,proliferation,cell cycle progress and *** expression level was obviously higher in SGC7901 and BGC823 than MKN28 and *** two cell lines were infected with LV-si-con and LV-CypB-si *** and cloney formation assays showed a significantly decreased rate of cell proliferation from the forth day or the fifth day in cells transfected with LV-CypB-si(P0.05).Down-regulation of CypB resulted in slightly decreased percentage of S phase and increased percentage of G_1(P0.05).These findings indicated that CypB could promote the G_1-S transition of gastric cancer *** addition,the expression of VEGF of BGC823 and SGC7901 transfected with CypB siRNA was reduced in comparison with the implicit control group and the blank control ***:Gene silencing of CypB decreases gastric cancer cells proliferation and in vivo *** findings indiccate CypB could be a potential biomarker and therapeutic target for gastric cancer.
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