Campylobacterjejuni in Musca domestica： An examination of survival and transmission potential in light of the innate immune responses of the house flies

作     者：Carson Gill Simon Bahrndorff Carl Lowenberger 

作者机构：Department of Biological Sciences Simon Fraser University 8888 University Drive Burnaby BC Canada National Food Institute Technical University of Denmark 2800 Kongens Lyngby Denmark 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2017年第24卷第4期

页      面：584-598页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 090602[农学-预防兽医学] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 09[农学] 0906[农学-兽医学] 0904[农学-植物保护] 0901[农学-作物学] 0713[理学-生态学] 

基　　金：Danish Council for Independent Research, Technology and Innovation, (11–116256) U.S. Department of Agriculture, USDA Natural Sciences and Engineering Research Council of Canada, NSERC, (RPG261940) Canada Research Chairs 

主　　题：antimicrobial peptides Campylobacter spp. Campylobacteriosis house fly innate immunity Musca domestica suppression subtractive hybridization （SSH） vector 

摘      要：The house fly, Musca domestica, has been implicated as a vector of Campy- lobacter spp., a major cause of human disease. Little is known whether house flies serve as biological amplifying hosts or mechanical vectors for Campylobacterjejuni. We in- vestigated the period after C. jejuni had been ingested by house flies in which viable C. jejuni colonies could be isolated from whole bodies, the vomitus and the excreta of adult M. domestica and evaluated the activation of innate immune responses of house flies to ingested C. jejuni over time. C. jejuni could be cultured from infected houseflies soon after ingestion but no countable C. jejuni colonies were observed 〉 24 h postingestion. We detected viable C. jejuni in house fly vomitus and excreta up to 4 h after ingestion, but no viable bacteria were detected 〉 8 h. Suppression subtractive hybridization identi- fied pathogen-induced gene expression in the intestinal tracts of adult house flies 4-24 h after ingesting C. jejuni. We measured the expression of immune regulatory （thor, JNK, and spheroide） and effector （cecropin, diptericin, attacin, defensing, and lysozyme） genes in C. jejuni-infected and -uninfected house flies using quantitative real time PCR. Some house fly factor, or combination of factors, eliminates C. jejuni within 24 h postingestion. Because C. jejuni is not amplified within the body of the housefly, this insect likely serves as a mechanical vector rather than as a true biological, amplifying vector for C. jejuni, and adds to our understanding of insect pathogen interactions.