RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

作     者：Shijun YAN He YAN Chaolin ZHANG Tongyan WANG Qingyuan YANG Zhe SUN Yan XIAO Feifei TAN Xiangdong LI Kegong TIAN 

作者机构：National Research Center for Veterinary Medicine College of Animal Science and Veterinary Medicine Henan Agricultural University 

出 版 物：《Frontiers of Agricultural Science and Engineering》 (农业科学与工程前沿（英文版）)

年 卷 期：2016年第3卷第1期

页      面：81-86页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 090601[农学-基础兽医学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 0836[工学-生物工程] 

基　　金：supported by grant from Major Science and Technology Program in Henan Province (131100110200) Innovation Scientists and Technicians Troop Construction Projects of Henan Province (142101510001) Talents Plan for Scientific and Technological Innovation in Henan Province (144200510002) Science and Technology Innovation team in Henan Province (C20130005) 

主　　题：pseudorabies virus RR1 and RR2 ribonucleotide reductase vaccine candidate 

摘      要：As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase(RR) in pseudorabies virus which have been extensively studied in mice. However,their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/g E/g I triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of g B-specific antibodies and virus neutralizing antibodies.However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.