Genotypic Characterization of Methicillin-resistant Staphylococcus aureus Isolated from Pigs and Retail Foods in China

作     者：WANG Wei LIU Feng ZULQARNAIN Baloch ZHANG Cun Shan MA Ke PENG Zi Xin YAN Shao Fei HU Yu Jie GAN Xin DONG Yin Ping BAI Yao LI Feng Qin YAN Xiao Mei MA Ai Guo XU Jin 

作者机构：School of Public Health Qingdao University Key Laboratory of Food Safety Risk Assessment Ministry of Health China National Center for Food Safety Risk Assessment Pharmaceutical DepartmentQingdao Hiser Medical Center College of Veterinary Medicine South China Agricultural University Kuiwen District Center for Disease Control and Prevention  6. State Key Laboratory for Infectious Disease Prevention and Control National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and Prevention 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学(英文版))

年 卷 期：2017年第30卷第8期

页      面：570-580页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100403[医学-营养与食品卫生学] 10[医学] 

基　　金：funded by the National Key R&D Program of China(2016YFD0401102)(the National Key Research and Development Program of China,the Ministry of Science and Technology of China) China Food Safety Talent Competency Development Initiative:CFSA 523 Program(1311613106702) 

主　　题：Methicillin-resistant Staphylococcus aureus Antimicrobial susceptibility Genetic diversity Pig Retail food 

摘      要：Objective To investigate the genotypic diversity of Methicillin-resistant Staphylococcus aureus(MRSA) isolated from pigs and retail foods from different geographical areas in China and further to study the routes and rates of transmission of this pathogen from animals to food. Methods Seventy-one MRSA isolates were obtained from pigs and retail foods and then characterized by multi-locus sequencing typing(MLST), spa typing, multiple-locus variable number of tandem repeat analysis(MLVA), pulsed-field gel electrophoresis(PFGE), and antimicrobial susceptibility testing. Results All isolated MRSA exhibited multi-drug resistance(MDR). Greater diversity was found in food-associated MRSA(7 STs, 8 spa types, and 10 MLVA patterns) compared to pig-associated MRSA(3 STs, 1 spa type, and 6 MLVA patterns). PFGE patterns were more diverse for pig-associated MRSA than those of food-associated isolates(40 vs. 11 pulse types). Among the pig-associated isolates, CC9-ST9-t899-MC2236 was the most prevalent clone(96.4%), and CC9-ST9-t437-MC621(20.0%) was the predominant clone among the food-associated isolates. The CC9-ST9 isolates showed significantly higher antimicrobial resistance than other clones. Interestingly, CC398-ST398-t034 clone was identified from both pig-and food-associated isolates. Of note, some community-and hospital-associated MRSA strains(t030, t172, t1244, and t4549) were also identified as food-associated isolates. Conclusion CC9-ST9-t899-MC2236-MDR was the most predominant clone in pigs, but significant genetic diversity was observed in food-associated MRSA. Our results demonstrate the great need for improved surveillance of MRSA in livestock and food and effective prevention strategies to limit MDR-MRSA infections in China.