Effect of Yang-activating and stasis-eliminating decoction from Traditional Chinese Medicine on intestinal mucosal permeability in rats with ulcerative colitis induced by dextran sulfate sodium

作     者：Shi Lijie Shi Lei Liu Leilei Feng Xiuxian Wang Jing Yang Qiang Chai Shiwei Gou Jing Zang Fenglin He Sufei 

作者机构：Department of Spleen and Stomach First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Department of Graduate School of Beijing University of Chinese Medicine Beijing 100029 China Department of Graduate School of Tianjin University of Traditional Chinese Medicine Tianjin 300000 China Department of Spleen and Stomach Shijiazhuang Traditional Chinese Medicine hospital Shijiazhuang 050000 China Department of Pharmacy First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Department of Clinical Laboratory First Teaching Hospital of Tianjin University of Traditional Chinese Medicine Tianjin 300193 China Department of Pathology Tianjin Medical University Cancer Institute and Hospital Tianjin 300060 China Department of Scientific Research Tianjin University of Traditional Chinese Medicine Tianjin 300073 China 

出 版 物：《Journal of Traditional Chinese Medicine》 (中医杂志（英文版）)

年 卷 期：2017年第37卷第4期

页      面：452-460页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 1005[医学-中医学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by National Natural Science Foundation of China Project:the Effect and Mechanism of Ulcerative Colitis Rats in the Intestinal Barrier Permeability by Huayutongyang Formula(No.81373848) 

主　　题：Colitis, ulcerative Epithelial cells Intestinal mucosa Permeability Occludin Dextran sulfate Removing blood stasis Activating Yang Medicine, Chinese Traditional 

摘      要：OBJECTIVE: To evaluate the effect of decoction prepared with Yang-activating and stasis-eliminating medicinals from Traditional Chinese Medicine(TCM) on the intestinal mucosal permeability in rats with ulcerative colitis induced by dextran sulfate sodium(DSS).METHODS: Totally 55 male Wistar rats(body weight of 170-190 g) were randomly divided into the blank group(n = 10) and the model duplication group(n = 45). The blank group was not intervened, while the other was modeled with 5% dextran sulfate sodium by gavaging in a dosage of4 m L per day to induce ulcerative colitis, a total of7 days. Then, the model rats were divided into model blank group, mesalazine group and TCM group,and each group was consisted of 15 rats. They were given retention enema 10 min with normal saline,mesalazine enema(0.036 g/m L), and Yang-activating and stasis-eliminating decoction [0.54 g/m L of a decoction boiled by Puhuang(Pollen Typhae), Xiebai(Bulbus Allii Macrostemonis) and Wulingzhi(Faeces Trogopteri)] for 10 days respectively. Afterwards,all of the rats were evaluated by disease activity index(DAI), histological changes of distal colon, expression of occludin protein and ultrastructure of intestinal epithelial cells. Furthermore, ratio of lactulose to mannitol(L/M) discharged in urine was ***: Comparing the results between TCM and model control groups, scores of DAI and histological lesions decreased significantly(P = 0.000 0.05).CONCLUSION: The decoction prepared with Yang-activating and stasis-eliminating TCM medicianls can restore intestinal mucosal epithelial cells and tight junctions the model rats with ulcerative colitis; it can redu