Short-term observations of the regenerative potential of injured proximal sensory nerves crossed with distal motor nerves

作     者：Xiu-xiu Zhang Yu-hui Kou Xiao-feng Yin Bao-guo Jiang Pei-xun Zhang 

作者机构：Department of Orthopedics and Trauma Peking University People's Hospital Beijing China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2017年第12卷第7期

页      面：1172-1176页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：supported by a grant from the Ministry of Science and Technology 973 Project Planning of China,No.2014CB542201 a grant from National High-Technology Research and Development Program of China(863 Program),No.SS2015AA020501 the National Natural Science Foundation of China,No.31571235,31571236,31271284,31171150 a grant from the Ministry of Education Innovation Team of China,No.IRT1201 the Educational Ministry New Century Excellent Talents Support Project of China,No.BMU20110270 a grant from the Ministry of Health of the Public Welfare Industry Special Scientific Research of China,No.201302007 

主　　题：nerve regeneration nerve remodeling peripheral nerve acetylcholinesterase staining muscle denervation neural anastomosis nerveconduit neural regeneration 

摘      要：Motor nerves and sensory nerves conduct signals in different directions and function in different ways.In the surgical treatment of peripheral nerve injuries,the best prognosis is obtained by keeping the motor and sensory nerves separated and repairing the nerves using the suture method.However,the clinical consequences of connections between sensory and motor nerves currently remain unknown.In this study,we analyzed the anatomical structure of the rat femoral nerve,and observed the motor and sensory branches of the femoral nerve in the quadriceps femoris.After ligation of the nerves,the proximal end of the sensory nerve was connected with the distal end of the motor nerve,followed by observation of the changes in the newly-formed regenerated nerve fibers.Acetylcholinesterase staining was used to distinguish between the myelinated and unmyelinated motor and sensory nerves.Denervated muscle and newly formed nerves were compared in terms of morphology,electrophysiology and histochemistry.At 8 weeks after connection,no motor nerve fibers were observed on either side of the nerve conduit and the number of nerve fibers increased at the proximal end.The proportion of newly-formed motor and sensory fibers was different on both sides of the conduit.The area occupied by autonomic nerves in the proximal regenerative nerve was limited,but no distinct myelin sheath was visible in the distal nerve.These results confirm that sensory and motor nerves cannot be effectively connected.Moreover,the change of target organ at the distal end affects the type of nerves at the proximal end.