Stereoselective interactions of chiral dipeptides on amylose based chiral stationary phases

作     者：Imran Ali Ashanul Haque Zeid A.Al-Othman Abdulrahman Al-Warthan Leonid Asnin 

作者机构：Department of Chemistry Jamia Millia Islamia Central University Department of Chemistry College of Science King Saud University Perm National Research Polytechnic University 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2015年第58卷第3期

页      面：519-525页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：the Department of Science and Technology, New Delhi, India (DST/INT/RFBR/P-147) the Russian Foundation of Basic Research, Russia (RFBR 13-03-92692) for financial assistance 

主　　题：dipeptide enantiomers amylose tris-(3 5-dimethylphenylcarbamate) chiral recognition mechanism elution order Auto Dock 4.2. 

摘      要：Dipeptides are stereo-specifically involved in several biological functions that are challenging to separate enantiomerically. Elution order of enantiomers is an important issue in chiral chromatography. Amylose tris-(3,5-dimethylphenylcarbamate) chiral stationary phase(CSP) is the best and most-widely-used CSP in chiral separations, but experimental data of enantiomeric separation of dipeptides on this CSP is lacking. Simulation studies were conducted to determine the order of elution and the chiral recognition mechanism of didpetides on this CSP. Results indicated that the docking energy of SR-enantiomers were higher than SS-antipodes. The range of docking energies for SR-enantiomers was -7.44 to -5.92 kcal/mol with CSP, but -7.15 to -5.87 kcal/mol for SS-stereoisomers. Therefore it is predicted that SS-enantiomer will elute first, followed by SR-antipode. Furthermore, hydrogen bondings, van der Waal s interactions and electrostatic interactions were observed among SR- and SSenantiomers and chiral grooves of CSP. The number of hydrogen bonds was one in each enantiomer binding except S-Ala-R-Tyr, which contained two hydrogen bonds. No hydrogen bond was found in S-Ala-R-Trp, S-Leu-S-Trp, and S-Leu-S-Tyr dipeptides bindings. The chiral recognition mechanisms dictate different strengths of stereoselective bindings of the enantiomers on CSP.