Chemoenzymatic synthesis of α2–3-sialylated carbohydrate epitopes

作     者：HUANG ShengShu YU Hai CHEN Xi 

作者机构：Department of Chemistry University of California One Shields Avenue Davis California 95616 USA 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第1期

页      面：117-128页

核心收录：

学科分类：0710[理学-生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 071007[理学-遗传学] 070303[理学-有机化学] 0901[农学-作物学] 0703[理学-化学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by NIH grants R01GM076360 and U01CA128442 

主　　题：carbohydrate chemoenzymatic synthesis sialic acid sialylation sialyltransferase 

摘      要：Sialic acids are common terminal carbohydrates on cell *** with internal carbohydrate structures,they play important roles in many physiological and pathological *** order to obtain α2–3-sialylated oligosaccharides,a highly efficient one-pot three-enzyme synthetic approach was *** *** α2–3-sialyltransferase (PmST1) involved in the synthesis was a multifunctional enzyme with extremely flexible donor and acceptor substrate *** acceptors,including type 1 structure (Galβ1–3GlcNAcβProN3),type 2 structures (Galβ1–4GlcNAcβProN3 and 6-sulfo-Galβ1– 4GlcNAcβProN3),type 4 structure (Galβ1–3GalNAcβProN3),type 3 or core 1 structure (Galβ1–3GalNAcβProN3) and human milk oligoscaccharide or lipooligosaccharide lacto-N-tetraose (LNT) (Galβ1–3GlcNAcβ1–3Galβ1–4GlcβProN3),were chemically *** were then used in one-pot three-enzyme reactions with sialic acid precursor ManNAc or ManNGc,to synthesize a library of naturally occurring β2–3-linked sialosides with different internal sugar *** sialylated oligosaccharides obtained are valuable probes for their biological studies.