Molecular tagging of a genic male-sterile gene in rice

作     者：CHEN Xuewei, LI Shigui, WANG Wenming, LI Hanyun, ZHOU Kaida & ZHU Lihuang1. Institute of Genetics, Chinese Academy of Science, Beijing 100101, China 2. Rice Research Institute, Sichuan Agricultural University, Chengdu 611130, China 

作者机构：Institute of Genetics Chinese Academy of Science Beijing China Rice Research Institute Sichuan Agricultural University Chengdu China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2001年第46卷第1期

页      面：66-69页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

主　　题：rice genie male-sterility molecular mapping molecular marker gene localization. 

摘      要：The sterility of Pingxiang male-sterile rice (Pins), possibly derided from a spontaneous mutation in Pingxiang fertile rice (Pmf), was previously reported to be controlled by a single dominant nuclear gene. It can be restored to fertility either by a dominant epistatic gene or by higher temperature treatment at the early stage of inflorescence development. In order to tag the genie male-sterile gene, Pms, Pmf and Ce 64, a cytoplasmic male-sterile restoring line without the epistatic gene for Pms, were used to construct mapping populations. Two segregation populations, (Pms/Ce 64) F1s (sterile plant)//Pmf F1 and Pms//(Pmf/Ce 64) F1 F1, were simultaneously developed. Subsequently, the genie male- sterile gene was mapped between a simple sequence length polymorphism marker, RM228, and a restriction fragment length polymorphism marker, G2155, with distances of 14.9 and 2.6 cM, respectively. The tagged dominant genie male-sterile gene is temporarily designated Ms-p.