The protection effect of β-CD on DNA damage induced by ultrafine TiO_2

作     者：ZHU RongRong,WANG ShiLong,SUN XiaoYu,ZHANG Rui & YAO SiDe School of Life Science and Technology,Tongji University,Shanghai 200092,China 

作者机构：1. School of Life Science and Technology Tongji University Shanghai 200092 China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2007年第50卷第2期

页      面：272-275页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by the National Natural Science Foundation of China (Grant No. 50572074) Shanghai Special Fund for Nano Materials (05nm05028) 

主　　题：β-CD ultrafine TiO2 plasmid DNA oxide damage bond reaction damage restrain 

摘      要：Under the photocatalysis of 365 nm ultraviolet radiation,ultrafine TiO2 caused the oxidative damage of Teasy plasmid DNA. The damage was determined by gel-electrophoresis. Then,a different dose of β-CD was added to the reaction,and the damage was restrained. The rate of damage restraining reached 97% when the mass of β-CD was 4 times as that of TiO2. Through UV scan and IR spectroscopy,it was found that the Ti-O of ultrafine TiO2 was bound with -OH of β-CD cavum and the -OH on the surface of ultrafine TiO2 disappeared,so the formation of ?OH was controlled. The ultrafine TiO2 has been widely used,but it was determined to be carcinogenic by some research. The protection effect of β-CD to DNA in the molecular level takes a new look on the surface modification of nano particles to decrease the toxic effect.