Staining Effect of Pomegranate Flower Extract on Human Blood Cells： First Results

作     者：Nilgun Guler Kusculuo Huseyin Benli 

作者机构：Department of Chemistry Technology Vocational School of Mustafa Ctkrtketoglu University of Erciyes Kayseri 38039 Turkey 

出 版 物：《Journal of Environmental Science and Engineering(A)》 (环境科学与工程（A）)

年 卷 期：2017年第6卷第5期

页      面：249-251页

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 082902[工学-木材科学与技术] 08[工学] 0829[工学-林业工程] 0836[工学-生物工程] 082203[工学-发酵工程] 0822[工学-轻工技术与工程] 083203[工学-农产品加工及贮藏工程] 

主　　题：Blood neutrophil pomegranate flower basophil eosinophil. 

摘      要：Pomegranate flower plant, popularly known using for the treatment of various diseases, was not investigated as a source of dye for cytological studies using human blood cells. The importance of this study is to appear dyeing result of pomegranate flower extract on human blood cells. The natural dye source was pomegranate flower known as roselle and potassium aluminum sulfate （alum = KAISO4＇ 12H20） was used as mordant or metal salt. Distilled water was used as solvent. Fresh, clean and air-dried flowers were extracted with distilled water at 100℃ for 30 minutes and then filtered. One drop blood from a healthy 20-year woman was spread as a peripheral on to ten plates and dried at nearly 25℃. These slides were stained by soaking in pomegranate flower extract with/without alum （KA1SO4·12H2O） at 100℃ for 60 minutes. Slides were washed with distilled water, dried and done microscobic examination. The different blood cells dyed dark orange in alum mordant media at 100 ℃. As a result, pomegranate flower has the capacity to use dyeing human blood cells such as eosinophil, basophil and neutrophil.