Lipopolysaccharide induces apoptosis of cytotrophoblasts by activating an innate immune reaction in vitro

作     者：LI Si-yang SHANG Tao LI Shu-juan RUI Guang-hai LI Qiu-ling 

作者机构：Department of Obstetrics and Gynecology 202th Hospital Shenyang 110003 China Department of Obstetrics and Gynecology Shengjing Hospital Chinese Medical University Shenyang 110004 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2007年第120卷第15期

页      面：1353-1359页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：lipopolysaccharide cytotrophoblast innate immunity apoptosis tumor necrosis factor α 

摘      要：Background Enhanced apoptosis of cytotrophoblasts in early pregnancy is associated with high risk of intrauterine growth retardation and preeclampsia, which are two common pregnant complications. Its etiological factors remain unclear. Cytotrophoblasts share some traits with innate immune cells and may show response to lipopolysaccharide. This study was conducted to demonstrate whether lipopolysaccharide has apoptosis-inducing effects on cytotrophoblast and the role of innate immune reaction in this process. Methods Cytotrophoblasts were isolated from eady pregnant villous tissues and cultured with serum-free medium. Subsequently, cytotrophoblasts were treated with lipopolysaccharide at the concentrations of 0 （control）, 25, 50, 100 and 200 ng/ml for 24 hours. Apoptosis of cytotrophoblasts was determined by light microscopy, Hoechst 33258 DNA staining with a fluorescent microscope, transmission electron microscope and annexin V-fluorescein isothiocyanate-cenjugated / propidium iodide （PI） staining with flow cytometry. Then expression of caspase-3 was detected by Western blot. Confocal immunofluorescence technique was used to detect tumor necrosis factor a expression in cytotrophoblasts. The levels of tumor necrosis factor a in the culture medium were detected by enzyme-linked immunosorbent assay. Results Under light, fluorescence microscope and transmission electron microscope, characteristic altemations of apoptosis in cytotrophoblasts were observed after lipopolysaccharide treatment. Flow cytometry results showed that lipopolysaccharide significantly increased apoptosis indexes of cytotrophoblasts. Significant statistical differences were found in the above groups （P≤0.01）. The mean relative densities of bands corresponding to caspase-3 were significantly increased in groups treated with lipopolysaccharide, as compared with the normal control （P〈0.001）. Tumor necrosis factor a expression was found to increase in cytotrophoblasts by confocal immunofluorescence