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A Transcriptomic Analysis of Physiological Significance of Hypoxia-inducible Factor-1α in Myogenesis and Carbohydrate Metabolism of Genioglossus in Mice

A Transcriptomic Analysis of Physiological Significance of Hypoxia-inducible Factor-1α in Myogenesis and Carbohydrate Metabolism of Genioglossus in Mice

作     者:Tong Hao Yue-Hua Liu Yuan-Yuan Li Yun Lu Hong-Yi Xu Hao Tong;Liu Yue-Hua;Li Yuan-Yuan;Lu Yun;Xu Hong-Yi

作者机构:Department of Orthodontics School and Hospital of Stomatology Tongji University Shanghai Engineering Research Center of Tooth Restoration and Regeneration Shanghai 200072 China Department of Orthodontics Shanghai Stomatological Hospital Shanghai 200001 China 

出 版 物:《Chinese Medical Journal》 (中华医学杂志(英文版))

年 卷 期:2017年第130卷第13期

页      面:1570-1577页

核心收录:

学科分类:090603[农学-临床兽医学] 0710[理学-生物学] 1002[医学-临床医学] 07[理学] 09[农学] 0906[农学-兽医学] 071007[理学-遗传学] 

基  金:This study was supported by grants from the National Natural Science Foundation of China (No. 81271192 and No. 81600897)  and the Shanghai Science and Technology Committee of China (No. 15140903500). 

主  题:Carbohydrate Metabolism Genioglossus Hypoxia-inducible Factor-1α Myogenesis Transcriptomic Analysis 

摘      要:Background: Chronic intermittent hypoxia is the most remarkable feature of obstructive sleep apnea/hypopnea syndrome and it can induce the change of hypoxia-inducible factor-1α (H IF-1α) expression and contractile properties in the genioglossus. To clarify the role of HIF-lot in contractile properties of the genioglossus, this study generated and compared high-throughput RNA-sequencing data from genioglossus between HIF-1α conditional knockout (KO) mice and littermate wild-type (WT) mice. Methods: KO mice were generated with cre-loxP strategy. Gene expression profile analysis was performed using gene enrichment analysis. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of differently expressed messenger RNAs were performed to identify the related pathways and biological lhnctions. Six differentially expressed genes (DEGs) were validated by qualitative reverse transcription polymerase chain reaction. Results: A total of 142 (77 upregulated and 65 downregulated) transcripts were found to exhibit statistically significant difference between the HIF-la-KO and WT mice. GO and KEGG analyses indicated that DEGs included genes involved in "skeletal muscle cell differentiation," "muscle organ development," "glucose metabolic process," "glycogen biosynthetic and metabolic process," etc. Conclusion: This study might provide evidence that H IF-lot affects the expression of multiple genes involved in the myogenesis, muscle developrnent, and carbohydrate metabolism through transcriptome analysis in conditional HIE-1α-KO mice.

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