Effects of chemical activation and season on birth efficiency of cloned pigs

作     者：MA YuFang1,2,LI Yan3,WEI HengXi1,LI QiuYan3,FANG Rui1,ZHAO Rui3,ZHANG Kun1,XUE Kai1,LOU YanKun1,DAI YunPing3,LIAN LinSheng2 & LI Ning1 1 State Key Laboratory of Agrobiotechnology,College of Biological Sciences,China Agricultural University,Beijing 100193,China 2 College of Animal Science and Technology,China Agricultural University,Beijing 100193,China 3 Beijing Genprotein Biotechnology Company,Beijing 100193,China 

作者机构：State Key Laboratory of Agrobiotechnology College of Biological Sciences China Agricultural University Beijing China College of Animal Science and Technology China Agricultural University Beijing China Beijing Genprotein Biotechnology Company Beijing China 

出 版 物：《Science China(Life Sciences)》 (中国科学（生命科学英文版）)

年 卷 期：2009年第52卷第7期

页      面：657-664页

核心收录：

学科分类：08[工学] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：Supported by National Key Basic Research and Development Program (China of Grant No. G200000161) 

主　　题：parthenogenetic activation chemical activation nuclear transfer pig season 

摘      要：The effects of chemical activation on birth efficiency of cloned pigs were studied by investigating the developmental process from porcine oocyte activation to birth of cloned *** different activation methods were used:(i) Electroporation(Ele);(ii) Ele followed by incubation with 6-dimethylaminopurine(6-DMAP);and(iii) Ele followed by a treatment with cycloheximide(CHX).In experiment 1,the rates of cleavage,developmental rates and cell number of porcine parthenogenetic(PA) embryos were investigated in the three treatment *** experiment 2,NT embryos produced by the three different activation treatments were compared for the rates of cleavage,development and cell ***,the effects of Ele and Ele+CHX activation methods on birth efficiency of cloned pigs were *** activated oocytes treated by combination activation generally showed a higher(P0.05) blastocyst rate and produced more expanded blastocysts than oocytes activated with *** rates of cleavage and total cell number of parthenotes were not significantly *** embryos activated with 6-DMAP developed into blastocyst and expanded blastocyst stages at a significantly(P0.05) higher rate than those treated with Ele,but the developmental capability was dramatically decreased in NT *** the CHX activation method,the NT embryo blastocyst rate was substantially(P0.05) increased although the production of expanded blastocysts was not significantly different from that by the other two *** birth rate of cloned pigs increased in the CHX group,though the rate was not significantly different from *** effects of season on developmental rate of the porcine PA embryos and birth rate of cloned pigs were also examined in our *** oocytes collected in the spring had higher developmental capabilities than those collected in the ***,no difference in birth rate of the cloned pigs was found between the oocytes collected in the two *** re