Bacteriological and electron microscopic examination of primary intrahepatic stones

作     者：Shuo-Dong Wu, Hong Yu and Ji-Mei Sun Department of General Surgery Department of Microbiology Center Second Affiliated Hos- pital China Medical University, Shenyang 110004, China 

作者机构：Department of General Surgery Second Affiliated Hospital (Shengjing Hospital) China Medical University Shenyang 110004 China. wushuodong@*** 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2006年第5卷第2期

页      面：228-231页

学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学] 

主　　题：cholelithiasis bacteria scan electron microscope transmission electron microscope biofilm 

摘      要：BACKGROUND: Primary intrahepatic cholelithiasis is usually combined with biliary tract infection. This research was undertaken to investigate the relationship between intrahepatic stones and biliary tract infection. METHODS: Thirty-five bile samples and 30 stones specimens were cultured for bacteria and 12 stones specimens were examined with a scan electron microscope (SEM) or a transmission electron microscope (TEM). RESULT: 94.2% bile samples and 96.7% stones specimens were positive in bacteria culture. Bacteria were found in stones under SEM and TEM. CONCLUSION: Bacteria in stones are associated with the infection of the biliary tract.