结肠直肠腺瘤与腺癌中p16、p53和p63的免疫组化表达研究

作     者：Carneiro F.P. Ramalho L.N.Z. Britto-Garcia S. 刘丽娜 

作者机构：Department of PathologyFaculty of Medicine of Ribeiro PretoUniversity of So PauloAv Bandeirantes 390014049-900 Ribeiro PretoSPBrazil Dr. 

出 版 物：《世界核心医学期刊文摘（胃肠病学分册）》 (Core Journals in Gastroenterology)

年 卷 期：2006年第2卷第10期

页      面：11-11页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：结肠直肠腺瘤 p16 p53 p63 绒毛状腺瘤 结肠直肠肿瘤 病理学参数 结肠直肠癌 肿瘤分化程度 

摘      要：PURPOSE: The aim of this study was to investigate the immunohistochemical expression of p16, p53, and p63 proteins according to some pathologic parameters related to colorectal adenomas and adenocarcinomas such as grade of dysplasia and histologic type. METHODS: Immunohistochemistry with the antibodies p16, p53, and p63 was performed in tubular, tubularvillous, and villous adenomas (n = 30) and in well, moderately, and poorly differentiated adenocarcinomas (n = 30). The p63-positive cases were submitted to double immunolabeling with the cytokeratin 5 (CK5). RESULTS: The p16 and p53 labelings were observed in some adenomas and adenocarcinomas but without any association with p63 expression, histologic type, or grade of differentiation of the neoplasm. P63 expression was found mainly in the villous adenomas and in the poorly differentiated adenocarcinomas. The poorly differentiated adenocarcinomas also exhibited coexpression of CK5 and p63. CONCLUSIONS: Despite both p16 and p53 having been detected in colorectal neoplasms, they were not related to the different histologic variables nor to the expression of p63. However, p63 expression was closely associated with villous adenomas and poorly differentiated adenocarcinomas. Thus, p63 may represent a marker of poor differentiation in colorectal neoplasms. The coexpression of p63 and CK5 observed in this study could be related to divergent differentiation during the development of colorectal cancer, although further studies are warranted to refine the understanding of this process.