Determination of sodium hyaluronate in pharmaceutical formulations by HPLC–UV

作     者：K.Ruckmani Saleem Z.Shaikh Pavne Khalil M.S.Muneera O.A.Thusleem 

作者机构：Department of Pharmaceutical Technology Anna University of Technology Department of Analytical Research & Development Jamjoom Pharmaceuticals 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2013年第3卷第5期

页      面：324-329页

学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 10[医学] 

基　　金：the authors wish to thank mr. noor sherrif  mr. anwer saeed  mr. mahmood jamjoom and colleagues in jamjoom pharma for their inspiration and motivation  which together are the true formulas for writing the scientific manuscript 

主　　题：Sodium hyaluronate Hyaluronic acid Size exclusion chromatography Derivatization Chromophore 

摘      要：A liquid chromatography （HPLC） method with UV detection was developed for determination of sodium hyaluronate in pharmaceutical formulation. Sodium hyaluronate is a polymer of disaccharides, composed of D-glucuronic acid and D-N-acetylglucosamine, linked via alternating 13-1, 4 and D-l, 3 glycosidic bonds. Being a polymer compound it lacks a UV absorbing chromophore. In the absence of a UV absorbing chromophore and highly polar nature of compound, the analysis becomes a major challenge. To overcome these problems a novel method for the determination of sodium hyaluronate was developed and validated based on size exclusion liquid chromatography （SEC） with UV detection. An isocratic mobile phase consisting of buffer 0.05 M potassium dihydrogen phosphate, pH adjusted to 7.0 using potassium hydroxide （10%） was used. Chromatography was carried out at 25 ~C on a BioSep SEC $2000, 300 mm ~ 7.8 mm column. The detection was carded out using variable wavelength UV-vis detector set at 205 nm. The compounds were eluted isocratically at a steady flow rate of 1.0 mL/min. Sodium hyaluronate retention time was about 4.9 min with an asymmetry factor of 1.93. A calibration curve was obtained from 1 to 38 g/mL （r〉0.9998）. Within-day % RSD was 1.0 and between-day % RSD was 1.10. Specificity/selectivity experiments revealed the absence of interference from excipients, recovery from spiked samples for sodium hyaluronate was 99-102. The developed method was applied to the determination of sodium hyaluronate in pharmaceutical drug substance and product.