Pleiotropic Effect of tatC Mutation on Metabolism of Pathogen Yersinia enterocolitica

作     者：ZHI-YANG SHI HUA WANG LING GU ZHI-GANG CUI LONG-FEI WU BIAO KAN BO PANG XIN WANG JIAN-GUO XU HUAI-QI JING 

作者机构：Jiangsu Provincial Center for Diseases Control and Prevention Nanjing 210009 Jiangsu China State Key Laboratory for Infectious Disease Prevention and Control National Institute of Communicable Diseases Prevention and Control Chinese Center for Diseases Control and Prevention Beijing 102206 China Laboratoire de Chimie Bacterienne IBSM CNRS Marseille F-13402 France 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2007年第20卷第6期

页      面：445-449页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 08[工学] 0836[工学-生物工程] 

主　　题：Yersinia enterocolitica TAT system Mutation 

摘      要：Objective To analyze the impact of depletion of the twin arginine translocation （TAT） system on virulence and physiology of Yersinia enterocolitica for a better understanding of its pathogenicity. Methods We constructed a △tatC：：Sp^R mutant of Yersinia enterocolitica by P1 phage mediated transduction using Escherichia coli K-12 △tatC：：Sp^R strain as a donor. Results A Pl-mediated genetic material transfer was found between the two species of enterobacteria, indicating a great potential of acquisition of antibiotic resistance in emergency of a new threatening pathogen by genetic material exchanges. Periplasmic trimethylamine N-oxidase reductase activity was detected in the wild type E enterocolitica strain and translocation of this enzyme was completely abolished by the △tatC：：Sp^R mutation. In addition, the △tatC：：Sp^R mutation showed a pleiotropic effect on the metabolism of E enterocolitica. However, the tat mutation did not seem to affect the mobility and virulence of Y. enterocolitica under the conditions used. Conclusion Unlike other pathogenic bacteria studied, the TAT system of E enterocolitica might play an important role in the pathogenic process, which is distinct from other pathogens, such as Pseudomonas aeruginosa and enterohemorrhagic E. coli O 157：H7.