Effects of Matrine on Bovine Papilloma Virus-infected C127 Cells

作     者：Jiang Xiao-wen Liu Lin Feng Xin-xin Liu Yan-yan Chen Xue-ying Feng Guo-feng Song Jian-chen Li Yan-hua Yu Wen-hui 

作者机构：College of Veterinary Medicine Northeast Agricultural University Harbin 150030 China Department of Veterinary Medicine Yanbian University Yanji 133002 Jilin China Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development Harbin 150030 China 

出 版 物：《Journal of Northeast Agricultural University(English Edition)》 (东北农业大学学报（英文版）)

年 卷 期：2017年第24卷第2期

页      面：51-58页

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 09[农学] 071007[理学-遗传学] 

基　　金：Supported by the Heilongjiang Provincial Education Department of Overseas Scientific Research Funded Projects(1251H006) 

主　　题：bovine papilloma virus C 127 cells matrine apoptosis 

摘      要：The aim of this study was to select drugs for prevention and treatment of bovine papilloma in vitro, using bovine papilloma virus（BPV-2） infected mouse mammary epithelial（C127） cell lines as the infectious cell model system. C127 cell shapes changed with BPV infection observed under light microscopy. C127 cell shapes changed with BPV infection observed under light microscopy. PCR was used to confirm BPV infected C127 cells. mR NA levels of matrine to BPV-2 infected C127 cells were detected by RT-PCR. Apoptosis of BPV-infected C127 cells treated with matrine was analyzed by flow cytometry. Results showed that 80 μg · mL-1 matrine had a direct virucidal and intervention effect of BPV-2. Matrine inhibited the proliferation of BPV-infected cells. Therefore, matrine might be regarded as a therapeutic drug candidate for BPV induced bovine papilloma disease treatment in future.