Causes of Deterioration and Changes of Aroma Components in Cigarette Raw Materials

作     者：Lizhi DANG Rui YUE Wenhan DONG Huaying WU Li SU Ting KOU Genhua YANG Yanqing DUAN 

作者机构：Research & Development CenterChina Tobacco Yunnan Industrial Co.Ltd. College of Plant ProtectionYunnan Agricultural University 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2017年第6卷第3期

页      面：10-15页

学科分类：08[工学] 0822[工学-轻工技术与工程] 

基　　金：Supported by the Project of the Key Laboratory of Cigarette Flavoring Components Research of China Tobacco Yunnan Industrial Co. Ltd.(2015539200340278) 

主　　题：Tobacco material Microbial populations Aroma components 

摘      要：The quality of the favoring agents and fragrances degenerated frequently during the storage and transportation process in a cigarette factory in Yunnan Province, causing huge economic losses. In order to ascertain the causes of deterioration, the microbial population, quantity and aroma components of the raw mate- rials were analyzed in the present study. The results showed that a total of 28 bacterial strains and one fungal strain were isolated and identified, belonging to 13 genera. The 29 strains were Bacillus sp. , Microbacterium sp. , Sphingomonas sp. , Leclercia sp. and Acinetobacter sp. isolated from normal ZY material; Bacil- lus sp. , Microbacterium sp. , Sphingomonas sp. , Brevundimonus sp. , Acinetobacter sp. , Agrobacterium sp. , Ralstonia sp. , Acetobacterium sp. , Saccharomyces sp. and Acidovorax sp. isolated from deteriorated ZY material; Agrobacterium sp. , Microbacterium sp. , Burkholderia sp. , Bacillus subtilis strain, Acetobacterium sp. and Leclercia sp. isolated from normal RZ material ; Agrobacterium sp. , Microbacterium sp. , Bacillus subtilis strain, Acetobacterium sp. , Lec/erc/a sp. , Enter- obacter sp. , Acinetobacter sp. and Saccharomyces sp. isolated from deteriorated RZ material. Microbacterium was the dominant genus by number in normal ZY ma- terial, and the population of this genus accounted for 55.6% of the total. In deteriorated ZY material, Acetobacterium, Bacillus and Saccharomyces were dominant genera, as the population of the three genera occupied 75.0% of the total. The total population of microorganisms in the deteriorated ZY material was 90 times of that in normal ZY material. In normal RZ material, the population of each of Microbacterium, Agrobacterium and Bacillus accounted for 25%, while that of each of Leclercia and Burkholderia accounted for 12.5%. In deteriorated RZ material, the population of Bacillus accounted for 65.0% of the total. The total population of the microorganisms in deteriorated RZ material was 29 times