Over-expression of the AtGA2ox8 gene decreases the biomass accumulation and lignification in rapeseed (Brassica napus L.)

作     者：Xiao-ying ZHAO Deng-feng ZHU Bo ZHOU Wu-sheng PENG Jian-zhong LIN Xing-qun HUANG Re-qing HE Yu-hong ZHUO Dan PENG Dong-ying TANG Ming-fang LI Xuan-ming LIU 

作者机构：Bioenergy and Biomaterial Research Center Institute of Life Science and Technology State Key Laboratory of Chemo/Biosensing and Chemometrics Hunan University Changsha 410082 China Institute of Tropical Bioscience and Biotechnology Chinese Academy of Tropical Agricultural Sciences Haikou 5 71101 China Academy of Seed Industry of Hunan Yahua Changsha 410001 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2010年第11卷第7期

页      面：471-481页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Project supported by the National High-Tech R & D Program (863) of China (No. 2007AA10Z127) the National Natural Science Foundation of China (No. 30800080) 

主　　题：Rapeseed AtGA2ox8 Biomass Lignification Gibberellins 

摘      要：Gibberellin 2-oxidase (GA 2-oxidase) plays very important roles in plant growth and development. In this study, the AtGA2ox8 gene, derived from Arabidopsis (Arabidopsis thaliana), was transformed and over-expressed in rapeseed (Brassica napus L.) to assess the role of AtGA2ox8 in biomass accumulation and lignification in plants. The transgenic plants, identified by resistant selection, polymerase chain reaction (PCR) and reverse-transcription PCR (RT-PCR) analyses, and green fluorescence examination, showed growth retardation, flowering delay, and dwarf stature. The fresh weight and dry weight in transgenic lines were about 21% and 29% lower than those in wild type (WT), respectively, and the fresh to dry weight ratios were higher than that of WT. Quantitative measurements demonstrated that the lignin content in transgenic lines decreased by 10%-20%, and histochemical staining results also showed reduced lignification in transgenic lines. Quantitative real-time PCR analysis indicated that the transcript levels of lignin biosynthetic genes in transgenic lines were markedly decreased and were consistent with the reduced lignification. These results suggest that the reduced biomass accumulation and lignification in the AtGA2ox8 over-expression rapeseed might be due to altered lignin biosynthetic gene expression.