Crystal structures, absolute configurations and molecular docking studies of naftopidil enantiomers as α1D-adrenoceptor antagonists

作     者：Wei Xu Junjun Huang Renwang Jiang Mu Yuan 

作者机构：College of Pharmacy Jinan University Pharmaceutical Research Center Guangzhou Medical University 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2017年第7卷第4期

页      面：496-501页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100701[医学-药物化学] 10[医学] 

基　　金：supported by grants from the National Science Foundation of Guangdong Province (No. S2013040014088) the Postdoctoral Science Foundation of Guangzhou City (Q188) partially supported by the Guangdong Major Scientific and Technological Special Project for New Drug Development (2013A022100029) 

主　　题：Naftopidil Crystal structure α1D-Adrenoceptor antagonists Binding mode Pharmacophore model 

摘      要：Chiral drug naftopidil(NAF), a specific α1D-adrenoceptor(AR) antagonist for the treatment of benign prostatic hyperplasia, was used in racemic form for several decades. Our recent work declared that NAF enantiomers showed the same antagonistic effects on the α1D-AR, but the binding mechanism of these two stereochemical NAF isomers to the α1D receptor remained unclear. Herein, we reported the crystallographic structures of optically pure NAF stereoisomers for the first time and unambiguously determined their absolute configurations. The crystal data of R and S enantiomers matched satisfactorily the pharmacophore model for α1D-selective antagonists. Based on the constructed α1D homology model,molecular docking studies shed light on the molecular mechanism of NAF enantiomers binding to α1D-AR. The results indicated that NAF enantiomers exhibited the very similar binding poses and occupied the same binding pocket.