月经周期中子宫内膜上皮和基质细胞的潜在干细胞活性

作     者：Schwab K.E. Gargett C.E. 李奎 

作者机构：Dr.Department of Obstetrics and Gynaecology Centre for Women's Health Research Monash University 246 Clayton Rd Clayton Vic. 3168 Australia 

出 版 物：《世界核心医学期刊文摘（妇产科学分册）》 (Core Journal in Obstetrics/Gynecology)

年 卷 期：2006年第2卷第2期

页      面：20-21页

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

主　　题：子宫内膜 基质细胞 月经周期 分泌期 子宫切除术 增殖期 非活动期 血清基 克隆分析 生长因子 

摘      要：Objective: To determine whether menstrual cycle stage or activity has an effect on the clonogenic activity of human endometrial epithelial and stromal cells. Design: Clonal analysis of human endometrial epithelial and stromal cells derived from full-thickness endometrium. Setting: University research laboratory. Patient(s): Twenty-six women of varying age and race undergoing hysterectomy for nonendometrial pathologies. Intervention(s): Full-thickness human endometrial tissue was dissociated into single cells. Epithelial and stromal cells were separated using magnetic beads, and cloning assays were performed in serum-containing or growth factor-supplemented serum-free medium. Main Outcome Measure(s): Clonogenic activity of epithelial and stromal cells. Result(s): Clonogenicity of epithelial and stromal cells did not vary significantly between proliferative, secretory, and inactive endometrium. However, epithelial and stromal cells did show a trend for greater numbers of clonogenic cells in secretory and proliferative endometrium respectively. A large variation between samples was observed, which may have masked any significant differences. Conclusion(s): We found that clonogenicity does not vary from the proliferative to secretory stage of the menstrual cycle, or between active, cycling and inactive endometrium for both epithelial and stromal cells. We have demonstrated for the first time that inactive endometrium contains clonogenic epithelial and stromal cells.