Sapium ellipticum(Hochst) Pax ethanol leaf extract modulates glucokinase and glucose-6-phosphatase activities in streptozotocin induced diabetic rats

作     者：Osasenaga Macdonald Ighodaro Oluseyi Adeboye Akinloye Regina Ngozi Ugbaja Samuel Olatunbosun Omotainse 

作者机构：Department of BiochemistryCollege of BiosciencesFederal University of AgricultureAbeokuta (FUNAAB) Department of BiochemistryFaculty of SciencesLead City University Department of Vertinary PathologyCollege of Vertinary MedicineFederal University of AgricultureAbeokuta (FUNAAB) 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2017年第7卷第6期

页      面：544-548页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Sapium ellipticum Glucose homeostasis Diabetes Glucokinase Glucose-6-phosphatase 

摘      要：Objective:To examine the effects of Sapium ellipticum(SE) leaf extract on the hepatic activities of glucokinase and glucose-6-phosphatase in streptozotocin-induced diabetic Wistar ***:STZ-induced diabetic Wistar rats(four groups,n = 8) were used in this *** was assessed at two different doses,400 and 800 mg/kg BW,in comparison with metformin(METF)(12 mg/kg BW) as a reference antidiabetic *** treatments were done orally(p.o),twice daily at 8 h interval for a period of 21 *** and glucose-6-phosphatase activities were respectively determined using standard *** and muscle glycogen contents were estimated as ***:STZ caused significant decrease in glucose-6-phosphatase activity and concomitant increase in glucokinase *** extract especially at 400 mg dosage significantly reversed the alterations by increasing glucokinase activity by 40.31% and inhibiting glucose-6-phosphatase activity by 37.29% compared to diabetic control ***,the effects were significantly lower than that of METF which enhanced glucokinase activity by94.76% and simultaneously inhibited glucose-6-phosphatase activity by 49.15%.The extract also improved hepatic glycogen level by 32.37 and 27.06% at 400 and 800 mg dosage ***-MS analysis of some SE fractions in dynamic MRM mode(using the optimized compound-specific parameters) revealed among other active compounds,the presence of amentoflavone,which has been associated with antidiabetic ***:The ability of SE extract to concurrently inhibit glucose-6-phosphatase and activate glucokinase in this study suggests that it may be a treatment option for type 2 diabetes patients,and the presence of amentoflavone in the plant extract may account for its anti-diabetic potential.