Heterologous expression of LamA gene encoded endo-β-1,3- glucanase and CO2 fixation by bioengineered Synechococcus sp. PCC 7002

作     者：Di Li Swati Yewalkar Xiaotao Bi Sheldon Duff Dusko Posarac Heli Wang Layne A. Woodfin Jan-Hendrik Hehemann Sheila C. Potter Francis E. Nano 

作者机构：School of Water Resources and Environment China University of Geosciences Beijing 100083 China Department of Chemical and Biological Engineering University of British Columbia Vancouve V6T 1Z3 Canada Department of Biochemistry and Microbiology University of Victoria Victoria V5Z 4H4 Canada 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2017年第11卷第2期

页      面：115-122页

核心收录：

学科分类：12[管理学] 1201[管理学-管理科学与工程(可授管理学、工学学位)] 08[工学] 

基　　金：加拿大自然科学与工程研究理事会(NSERC)项目 

主　　题：Synechococcus sp. PCC 7002Thermotoga maritimaLamA geneEndo-β-1 3-glucanaseCO2 fixation 

摘      要：The gene for the catalytic domain of thermostable endo-β-1,3-glucanase （laminarinase） LamA was cloned from Thermotoga maritima MSB8 and heterologously expressed in a bioengineered Synechococcus sp. PCC 7002. The mutant strain was cultured in a photobioreactor to assess biomass yield, recombinant laminarinase activity, and CO2 uptake. The maximum enzyme activity was observed at a oH of 8.0 and a temoerature of 70℃. At a CO2 concentration of 5%, we obtained a maximum specific growth rate of 0.083 h^-1 a biomass productivity of 0.42 g· L^-1·d^-1 a blomass concentration of 3.697 g.L^-1 , and a specific enzyme activity of the mutant strain of 4.325 ***^- 1 dry mass. All parameters decreased as CO2 concentration increased from 5% to 10% and further to 15% CO2, except enzyme activity, which increased from 5% to 10% CO2. However, the mutant culture still 1 1 grew at 15% CO2 concentration, as reflected by the blomass productwlty （0.26 g.L .d ）, biomass concentration （2.416 g.L^- 1）, and specific enzyme activity （3.247 ***^-1 dry mass）.