Mapping QTLs for Heat Tolerance in Rice (Oryza sativa L. ) at Heading Stage Using Chromosome Segment Substitution Lines

作     者：Changquan ZHANG Fei CHEN Ran HONG Qianfeng LI Minghong GU Qiaoquan LIU 

作者机构：College of AgronomyYangzhou University 

出 版 物：《Agricultural Biotechnology》 (农业生物技术（英文版）)

年 卷 期：2017年第6卷第2期

页      面：15-18页

学科分类：09[农学] 0901[农学-作物学] 

基　　金：Supported by the National Natural Science Foundation of China(31561143008,31401354) Jiangsu Provincial Natural Science Foundation(BK20140484) Doctoral Fund of Ministry of Education of China(20133250120001) 

主　　题：Rice (Oryza sativa L. ) Chromosome segment substitution lines (CSSLs) Quantitative trait loci (QTLs) Heat tolerance 

摘      要：In the present study, a japonica rice （ Oryza sativa L. ） variety Nipponbare, an indica variety 9311 and a set of chromosome segment substitution lines （CSSLs） which were generated using Nipponbare as the recipient parent and 9311 as the donor parent were used as the experimental materials. The CSSLs were grown in 2012 （normal temperature condition） and 2013 （high temperature condition） in Yangzhou, Jiangsu, and were used to map the quantitative trait loci （QTLs） for heat tolerance, based on the heat tolerance index [ （The seed setting rate under normal temperature condition -The seed setting rate under high temper- ature condition） / The seed setting rate under normal temperature condition]. As a result, three QTLs related to heat tolerance in rice were mapped on chromo- somes 2, 4 and 12, respectively. They had LOD （logarithm of rntds） scores of 2.56, 4.02 and 2.79, and contributian rates of 4.95%, 7.99% and 5.44%. Among them, qHT12.1 showed positive effect, while qHT2.1 and qHT4. t showed negative effect on heat tolerance. The results lay a foundation for the fine mapping and cloning of the QTLs and genes related to heat tolerance, and for the breeding of heat-tolerant rice varieties.