Preliminary study on investigation of zoonotic visceral leishmaniasis in endemic foci of Ethiopia by detecting Leishmania infections in rodents

作     者：Wossenseged Lemma Asrat Bizuneh Habte Tekie Habtamu Belay Hirut Wondimu Aysheshm Kassahun Welelta Shiferaw Meshesha Balkew Ibrahim Abassi Gad Baneth Asrat Hailu 

作者机构：Department of Medical ParasitologySchool of Biomedical and Laboratory SciencesCollege of Medicine and Health SciencesUniversity of Gondar Department of MicrobiologyImmunology and ParasitologySchool of MedicineAddis Ababa University Department of Zoological SciencesCollege of Natural ScienceAddis Ababa University Department of ParasitologyFaculty of ScienceCharles University in Prague Aklilu Lemma Institute of PathobiologyAddis Ababa University Department of Microbiology and Molecular Geneticsthe Institute for Medical Research Israel-Canadathe Kuvin Centre for the Study of Infectious and Tropical Diseasesthe Hebrew University-Hadassah Medical School School of Veterinary MedicineHebrew University 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2017年第10卷第4期

页      面：396-400页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：supported by the Bill and Melinda Gates Foundation Global Health Program(Grant number OPPGH5336) Gondar University 

主　　题：Zoonotic visceral leishmaniasis Phlebotomus orientalis Phlebotomus martini Leishmania donovani Reservoir hosts Ethiopia 

摘      要：Objective:To investigate the zoonotic visceral leishmaniasis(ZVL) by identification of the most probable reservoir hosts using parasite isolation and analysis of a possible transmission dynamics of the disease in extra-domestic agricultural fields and rural ***:Rodents were collected from selected study sites in kala-azar endemic areas based on information for localities of kala-azar cases for screening of Leshmania infections using parasitological,serological and polymerase chain reaction(PCR) from March.2013 to January,***(Clorketam Veterinary) was used to anaesthesize the rodents according the prescribed dosage(average 2 mg/kg for intra-venous route).The blood obtained using sterile needle was dropped into sterile filter paper and allowed to air dry before sealing in plastic *** tissues from liver,spleen and skin were macerated in Locke s solution before transferring them into NNN *** and touch smears of liver,spleen,skin and bone marrow were prepared for fixing using methanol and staining by Giemsa stain for *** tissues were also ascd for DNA extractions and PCR amplification of Leishmania ***:A total of 335 rodents(13 species) were analyzed by sampling internal *** infection rate by PCR was 11.1%(6/54) for Arvicanthis nilothicas compared to 17.6%(3/17) and 12.5%(2/16) for Acomys cahirinus and Tarera(C) robustus *** all the infections were found from bone marrow samples(8/48 or 16.7%) compared with 1/91(1.1%) liver,2/87(2.2%) spleen and 0/87(0%) *** all study sites with past human VL cases,rodents and proved vectors shared similar ***:Leishmania donovani might circulate among different species of rodents in kala-azar endemic lowlands and valleys of Ethiopia by Phlebotamus orientalis and Phlebotomus *** studies to substantiate the preliminary data on the possible role of these rodents arc urgently needed.