Reprogramming somatic cells to cells with neuronal characteristics by defined medium both in vitro and in vivo

作     者：Songwei He Yiping Guo Yixin Zhang Yuan Li Chengqian Feng Xiang Li Lilong Lin Lin Guo Haitao Wang Chunhua Liu Yi Zheng Chuanming Luo Qiang Liu Fuhui Wang Hao Sun Lining Liang Lingyu Li Huanxing Su Jiekai Chen Duanqing Pei Hui Zheng 

作者机构：CAS Key Laboratory of Regenerative BiologyGuangdong Provincial Key Laboratory of Stem Cell and Regenerative MedicineGuangzhou Institutes of Biomedicine and HealthChinese Academy of Sciences#190 Kaiyuan AvenueScience CityGuangzhou 510530China State Key Laboratory of Quality Research in Chinese MedicineInstitute of Chinese Medical SciencesUniversity of MacaoMacaoChina Anhui UniversityHefei 230601China 

出 版 物：《Cell Regeneration》 (细胞再生（英文）)

年 卷 期：2015年第4卷第1期

页      面：123-131页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was supported by“Strategic Priority Research Program of the Chinese Academy of Sciences(XDA01020302)” the“National Natural Science Foundation of China(31422032,31421004)” the“Guangdong Natural Science Foundation(2014A030308002)” the“Guangdong Science and Technology Planning Project(2013B010404040),” the“Guangzhou Health Care Collaborative Innovation Program(201508020250)” We sincerely thank Dr.Chen Ling(mouse macrophages) Dr.Wang Lihui(HFFs)in GIBH,and Dr.Peng Xiang(BM-hMSCs)in Sun Yat-Sen University for providing cells 

主　　题：Neurons Somatic cells Astrocytes Trans-differentiation Defined medium 

摘      要：Background:Currently,direct conversion from somatic cells to neurons requires virus-mediated delivery of at least one transcriptional factor or a combination of several small-molecule *** of transcriptional factors may affect genome stability,while small-molecule compounds may require more evaluations when applied in ***,a defined medium with only conventional growth factors or additives for cell culture is desirable for inducing neuronal ***:Here,we report that a defined medium(5C)consisting of basic fibroblast growth factor(bFGF),N2 supplement,leukemia inhibitory factor,vitamin C(Vc),andβ-mercaptoethanol(βMe)induces the direct conversion of somatic cells to cells with neuronal *** of 5C medium converted mouse embryonic fibroblasts(MEFs)into TuJ+neuronal-like cells,which were capable of survival after being transplanted into the mouse *** same 5C medium could convert primary rat astrocytes into neuronal-like cells with mature electrophysiology characteristics in vitro and facilitated the recovery of brain injury,possibly by inducing similar conversions,when infused into the mouse brain in ***,5C medium could also induce neuronal characteristics in several human cell ***:In summary,this 5C medium not only provides a means to derive cells with neuronal characteristics without viral transfection in vitro but might also be useful to produce neurons in vivo for neurodegenerative disease treatment.