Anti-inflammatory effect of amurensin H on asthma-like reaction induced by allergen in sensitized mice

作     者：Yi-tang LI~2 Chun-suo YAO~3 Jin-ye BAI~2 Man LIN~3 Gui-fang CHENG~(2,4)2 Department of Pharmacology,Institute of Materia Medica,Peking Union Medical College and Chinese Academy of Medical Sciences,Beijing 100050,China 3 Department of Photochemistry,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China 

作者机构：Department of Pharmacology Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences Beijing China Department of Photochemistry Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China 

出 版 物：《Acta Pharmacologica Sinica》 (中国药理学报(英文版))

年 卷 期：2006年第27卷第6期

页      面：735-740页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 1002[医学-临床医学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Project supported by grants from the Institute of Materia Medica Peking Union Medical College and Chinese Academy of Medical Sciences(№231766) 

主　　题：amurensin H asthma ovalbumin tumor necrosis factor-α Th2 cells cytokines 

摘      要：Aim:To explore the anti-inflammatory effects of amurensin H on asthma-like reac-tion induced by allergen in sensitized ***:BALB/c mice were sensi-tized by ovalbumin(OVA,ip)on d 0 and d 14 and challenged with 1% OVA on d 18to *** developed airway eosinophilia,mucus hypersecretion,and elevationin cytokine *** were administered amurensin H orally at the doses of 49,70,or 100 mg/kg once every day from d 15 to the last *** lavagefluid(BALF)were collected at 24 h and 48 h after the last OVA *** tumor necrosis factor-α(TNF-α),interleukin 4(IL-4),interleukin 5(IL-5),andinterleukin 13(IL-13)in BALF were measured using ELISA *** counts of macrophages,lymphocytes,neutrophils and eosinophils were per-formed in 200 cells per slide(one slide per animal).Lung tissue sections of 6-μmthickness were stained with Mayer’s hematoxylin and eosin for assessment of cellinfiltration,mucus production,and tissue ***:Oral administrationof amurensin H significantly inhibited OVA-induced increases in total cell counts,eosinophil counts,and TNF-α,IL-4,IL-5 and IL-13 levels in *** addition,amuresin H dramatically decreased OVA-induced lung tissue damage and ***:Amurensin H may have therapeutic potential for thetreatment of allergic airway inflammation.