Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method
Studies on Intestinal Transport of Ginsenoside Compatibility with Veratrum nigrum via Caco-2 Cell Monolayer Model Coupled with UPLC-ESI-MS Method作者机构：College of Pharmacy Jilin University Changchun 130021 P. R. China Changchun Institute of Applied Chemistry Chinese Academy of Sciences Changchun 130022 P. R. China
出 版 物：《Chemical Research in Chinese Universities》 (高等学校化学研究（英文版）)
年 卷 期：2015年第31卷第6期
页 面：914-918页
核心收录：
学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0906[农学-兽医学] 0901[农学-作物学] 090102[农学-作物遗传育种]
基 金：Supported by the National Natural Science Foundation of China(Nos.81073040 81274046 81173507) the Jilin Provincial Science and Technology Agency-funded Projects China(No.20150414040GH) and the National Basic Research Program of China(Nos.2011 CB505300 2011 CB505305)
主 题：High performance liquid chromatography-electrospray ionization-mass spectroscopy(HPLC-ESI-MS) Panax ginseng Ginsenoside Veratrum nigrum Transport Caco-2 cell monolayer model
摘 要：The Caco-2 cells have been recognized as effective tools to be applied to imitating the drug absorption in human intestine for the transport of drug. Herein, Caco-2 cell monolayer model was used to study the transport of the ginsenoside compatibility with Veratrum nigrum in different proportions. A specific high performance liquid chro- matography-electrospray ionization-mass spectrometry（HPLC-ESI-MS） method was developed for the semiquantita- tive determination of ginsenoside in intestinal transport with Dioscin as an internal standard. For the Caco-2 model constructed, two influencing factors were investigated, including time and concentration. The results suggest that the absorption of ginsenoside Re, Rgl, Rbl, Rc, Rb2 and Rd are time- and concentration-dependent and the excretions of Rbl, Rc, Rb2 and Rd have a relatronship with some transport proteins. The bioavailability of the ginsenosides has reduced compared to the single Panax ginseng extract when compatibility with a certain amount of Veratrum nigrum.
维普期刊数据库