Surfactant 4-nonylphenyl-polyethylene glycol stimulates reactive oxygen species generation and apoptosis in human neuroblastoma cells

作     者：Tanana Zerin Hoyeon Song Hyowook Gil Saeyong Hong 

作者机构：Department of MicrobiologyCollege of MedicineSoonchunhyang UniversitySouth Korea. Pesticide Poisoning CenterSoonchunhyang University Cheonan HospitalSouth Korea 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2017年第29卷第3期

页      面：262-268页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 

基　　金：supported by the Cooperative Research Program for Agriculture Science and Technology Development of the Rural Development Administration South Korea(Project no.PJ01083201) 

主　　题：ApoptosisCytochrome cCaspase 3/7 Caspase 9 Nonionic surfactantOxidative stress 

摘      要：The formulation of most pesticides is proprietary and individual components are therefore not generally known. In a preliminary study, we identified six compounds that are often present in pesticides, of which 4-nonylphenyl-polyethylene glycol （NP-40） was found to be the most toxic. In this study, we investigated the toxicity of NP-40 and underlying mechanism in neuronal SK-N-SH cells. Exposure to NP 40 at concentrations higher than 60 μmol/L for 24 hr decreased cell viability. The cytotoxicity of NP-40 was time- and concentration-dependent. Nuclear fragmentation and chromatin condensation were apparent starting at 50 μmol/L NP-40, and increased at higher concentrations. The expression of apoptotic factors including p53 and B-cell lymphoma （Bcl）-2-associated X protein was upregulated, while that of the anti-apoptotic marker Bcl-2 was downregulated at 80 μmol/L NP-40. Cytochrome c release was observed from 80 to 100 μmol/L by confocal microscopy. Caspase-9 and -3/7 activities increased according to concentration, and fluorescence-activated cell sorting analysis showed that apoptosis was induced at 50 μmol/L and was increased at 80 μmol/L. Our findings indicate that NP-40 stimulates the mitochondrial-mediated apoptosis pathway and reactive oxygen species production in a concentration-dependent manner, and suggest that antioxidant administration may be an effective treatment for patients with acute NP-40 poisoning.