Expression of Nogo-A mRNA after injury of the rat central nervous system
Expression of Nogo-A mRNA after injury of the rat central nervous system作者机构:Department of Neurosurgery the First People's Hospital of Shunde Foshan 528300 Guangdong Province China
出 版 物:《Neural Regeneration Research》 (中国神经再生研究(英文版))
年 卷 期:2008年第3卷第12期
页 面:1368-1371页
核心收录:
学科分类:0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学]
基 金:the Medical Research Foundation of Guangdong Province No.A2005707
主 题:central nervous system neural regeneration Nogo-A
摘 要:BACKGROUND: Nogo protein has been identified as an inhibitor of axonal growth, which was highly expressed in central nervous system; however, there are only a few studies on changes of Nogo-A expression following central nervous system injury. OBJECTIVE: To investigate the dynamic expression of Nogo-A mRNA after rat central nervous system injury. DESIGN: Randomized controlled animal study. MATERIALS: Thirty-five rats were randomly divided into two groups, normal animal group (n = 5) and model group (n = 30). The model group was then divided into six subgroups at six time points: 12, 24 hours and 3, 9, 15, and 21 days post-injury, with five rats in each subgroup. METHODS: The left parietal lobe of rats was contused by free-fall strike, and total RNA was extracted from the entire brain tissue. Semi-quantitative RT-PCR was used to detect Nogo-A mRNA expression, and the ratio between expression of the target gene and glyceraldehyde phosphate dehydrogenase was used to determine the relative expression level. MAIN OUTCOME MEASURES: To determine whether Nogo-A mRNA expression was higher than usual following brain injury. RESULTS: The level of Nogo-A mRNA started to increase 12 hours after injury (P 〈 0.05) and decreased slightly by 24 hours post-injury. Expression increased again on day 3 and reached a peak on day 9. Nogo-A mRNA expression started to decrease on day 15, and then decreased to normal levels at days 21 (P 〉 0.05). CONCLUSION: After injury of the central nervous system, Nogo-A may play a pivotal role in obstructing regeneration of the nerve.