Proliferation and differentiation into endothelial cells of human bone marrow mesenchymal stem cells (MSCs) on poly DL-lactic-co-glycolic acid (PLGA) films

作     者：YUE Huimin ZHANG Lei WANG Yunfang LIANG Feng GUAN Lidong Li Shaoqing YAN Fang NAN Xue BAI Cixian LIN Feng YAN Yongnian PEI Xuetao 

作者机构：Department of Stem Cell Biology Institute of Transfusion Medicine Academy of Military Medical Sciences Beijing 100850 China Research Center of Laser Rapid Forming Mechanical Department of Tsinghua University Beijing 100084 China These authors contributed equally to this work. 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2006年第51卷第11期

页      面：1328-1333页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 08[工学] 0836[工学-生物工程] 

基　　金：2001CB509906,2005CB522702 2005AA219010 

主　　题：MSCs PLGA 内皮细胞 内皮化 维管组织工程 

摘      要：The functional realization is the most important problem in vascular tissue engineering. The small-caliber blood vessel substitutes are prone to thrombi, which results in functional loss of blood vessels. However, this is probably due to the imper- fection of endothelial layer in the substitutes. In this study, MSCs were seeded on a series of porous PLGA films with various porosity and pore size made by sodium chloride (NaCl) particulate leaching, and cell proliferation on each film was inspected. The film made of the 75% (w/w) particulate proportion and 30―50 μm pore size maximized the proliferation rate and was chosen as the scaffolds for the differentia- tion of MSCs into endothelial cells. The induced cells expressed endothelial cells specific Flk-1, Ⅷ factor and CD34, possessed endothelial cells specific Weible-palade (W-P) body, and had the abilities of ingesting low density lipoprotein and secreting prostacyclin (PGI2). The results show that MSCs not only have the ideal biological compatibility with the porous PLGA films, but also have the potency of dif- ferentiating into functional endothelial cells, whichshould facilitate the endothelialization in vascular tissue engineering.