Effect of Berberine on the mRNA Expression of Nitric Oxide Synthase(NOS) in Rat Corpus Cavernosum

作     者：谭艳 明章银 汤强 蒋兆键 胡本容 向继洲 

作者机构：DepartmentofPharmacologySchoolofBasicMedicalSciencesTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China DepartmentofPharmacologySchoolofBasicMedicalSciencesTongjiMedicalCollegeHuazhongUniversityofScienceandTechnologyWuhan430030China 

出 版 物：《Journal of Huazhong University of Science and Technology(Medical Sciences)》 (华中科技大学学报（医学英德文版）)

年 卷 期：2005年第25卷第2期

页      面：127-130页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：This project was supported by a grant from the National Natural Science Foundation of China (No. 30171151) 

主　　题：berberine nitric oxide synthase nitric oxide corpus cavernosum erectile dysfunction 

摘      要：Summary: In order to further investigate the mechanisms of action of berberine (Ber), we assessed the effects of Ber on the mRNA expression of nitric oxide synthases (NOS) in rat corpus cavernosum. After incubation with Ber for 1 or 3 h respectively, the levels of NOS mRNA were examined by reverse transcription polymerase chain reaction (RT-PCR). Our results showed that there were iNOS and eNOS mRNA expressions in rat corpus cavernosum. Ber enhanced eNOS mRNA expression in rat penis, but exhibited no effect on the expression of iNOS mRNA (P0.05). The present study indicated that the relaxation of Ber involved the NO-cGMP signal thansduction pathway. The enhancing effect of Ber on eNOS mRNA expression might associated with its relaxation of corpus cavernosum.