Construction and identification of recombination expression vector Ksp-Cadherin-Gpx1-Klk1

作     者：解立怡 薛武军 项和立 麻孙凯 

作者机构：Renal Disease Centerthe First Affiliated Hospital Medical School of Xi'an Jiaotong University Renal Disease Center the First Affiliated Hospital Medical School of Xi'an Jiaotong University Shanghai Research Center for Bio model Organism 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2008年第20卷第4期

页      面：217-220,255页

学科分类：1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China (No.30471640) 

主　　题：Gpx1 Klk1 Ksp-cadherin ischemic-reperfusion injury 

摘      要：Objective To construct and identify the Gpx1-Klk1 vector which contains kidney-specific promoter (Ksp-cadherin). Methods Through PCR amplification, the human Gpx1, Klk1, and Ksp-cadherin cDNA were obtained by taking Gpx1 cDNA, Klk1 cDNA, and Ksp-cadherin BAC as templates. After being testified, the PCR products were inserted into the expressive vector pIRES-EGFP step-by-step to produce a recombinant vector Ksp-cadherin-Gpx1-Klk1. This vector was examined by restriction enzyme digestion and sequence analysis. Results The recombinant expressive vector Ksp-cadherin-Gpx1-Klk1 was successfully constructed. Conclusion The construction of the recombinant vector Ksp-cadherin-Gpx1-Klk1 laid foundations for investigations in establishing transgenic animal models, the over-expression of Gpx1 and Klk1 in mammal kidney, and gene therapy for ischemia-reperfusion injury during kidney transplantation.