Photosynthesis and biochemical responses to elevated O_3 in Plantago major and Sonchus oleraceus growing in a lowland habitat of northern China

作     者：Benying Su Meihua Zhou Hong Xu Xiujie Zhang Yonggeng Li Hua Su Bao Xiang 

作者机构：State Key Laboratory of Environmental Criteria and Risk AssessmentChinese Research Academy of Environmental Sciences State Key Laboratory of Vegetation and Environmental ChangeInstitute of BotanyChinese Academy of Sciences 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2017年第29卷第3期

页      面：113-121页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 07[理学] 070602[理学-大气物理学与大气环境] 09[农学] 0903[农学-农业资源与环境] 0706[理学-大气科学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No.30900200) the China Postdoctoral Science Foundation (No.2015M571087) 

主　　题：Biochemical analysisHerbaceous speciesGas exchangeOzoneSemi arid grassland 

摘      要：A field experiment was carried out to compare the responses to ozone （03） in two common herbaceous plant species, Plantago major L. and Sonchus oleraceus L., by building open-top growth chambers in situ to simulate 03 stress （＋03, 85 ± 5 ppb, 9 hr/day for 30 days） in a lowland habitat in Inner Mongolia, Northern China. Responses to 03 of gas exchange, chlorophyll a fluorescence, leaf pigment content, andoxidant capability, soluble protein content, membrane lipid peroxidation and dark respiration （Rd） were analyzed. Results showed that elevated 03 exposure significantly reduced the light-saturated net photosyn- thesis （PNsat）, stomata] conductance （gs） and transpiration rate （E） in both species. Although non-significant interactive effect between species and 0s on PNsat was analyzed, the reduction in PNsat in S. oleraceus might be due primarily to the higher fraction of close PSII reaction centers and impaired activities of plant mesophyll cells as evidences by decreased maximum efficiency of PSII photochemistry after dark adapted state （Fv/Fm） and unchanged intercellular CO2 concentration （Ci）. Besides, biochemical analysis showed that S. oleraceus had lower antioxidant ability compared to P. major. As a result, S. oleraceus was damaged to the larger extent in terms of lipid peroxidation and visible O3 injury, indicating that S. oleraceus was more sensitive to O3 than P. major. Our results indicated that wild herbaceous plant species growing in a lowland habitat in sandy grassland were sensitive to O3 stress and S. oleraceus can be considered as one of the bio-indicators for high O3 concentration in semi-arid -rassland of northern China.