Cultivable Sponge-Associated Actinobacteria from Coastal Area of Eastern Mediterranean Sea

作     者：Ozlem Oner Güner Ekiz E.Esin Hames Volkan Demir Ozkan Gübe F.Can Ozkaya M.Baki Yokes Atac Uzel Erdal Bedir 

作者机构：Department of BiotechnologyGraduate School of Natural and Applied SciencesEge UniversityBornovaIzmirTurkey Department of BioengineeringFaculty of EngineeringEge UniversityBornovaIzmirTurkey Institute of Marine Sciences and Managementİstanbul UniversityVefaİstanbulTurkey Department of Molecular Biology and GeneticsFaculty of Arts and SciencesHalic UniversitySıracevizler Cd.No:29 BomontisisliIstanbulTurkey Department of BiologyFaculty of ScienceEge UniversityBornovaIzmirTurkey 

出 版 物：《Advances in Microbiology》 (微生物学（英文）)

年 卷 期：2014年第4卷第6期

页      面：306-316页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：supported by Scientific and Technological Research Council of Turkey(TUBİTAK)with SBAG-109S361project number 

主　　题：Sponge-Associated Actinobacteria Streptomyces Nocardiopsis Antimicrobial Activity 

摘      要：In this study, 16 marine sponge samples collected from Eastern Mediterranean Sea were used for the isolation of sponge-associated Actinobacteria. Different media and pre-treatment methods were applied for the isolation experiments. In total, 7578 actinomycetes colonies were observed on the primary isolation plates, and 20 representative isolates were selected for further studies based on their morphologies and chemical profiles. These isolates were investigated for their antimicrobial activities, seawater requirements and 16S rRNA gene sequences. The phylogenetic analysis revealed that 80% of the isolated Actinobacteria was a member of the Streptomyces genus. All the isolates showed no absolute requirement for seawater. Fifteen of the selected isolates exhibited antimicrobial activity against methicillin resistant Staphylococcus aureus and vancomycin resistant Enterococcus faecium, whereas 3 isolates were active against Candida albicans.