2,5-Dihydro-2,4,5-Trimethylthiazoline (TMT)-Induced Neuronal Activation Pattern and Behavioral Fear Response in GAD67 Mice

作     者：Kathrin Janitzky Olaf Prellwitz Herbert Schwegler Yuchio Yanagawa Thomas Roskoden 

作者机构：Department of Neurology University of Magdeburg Magdeburg Germany Leibniz Institute of Neurobiology Magdeburg Germany Department of Orthopedics and Traumatology Clinical Center in the Pfeiffersche Stiftungen Magdeburg Germany Institute of Anatomy University of Magdeburg Magdeburg Germany Department of Genetic and Behavioral Neuroscience Gunma University Maebashi Japan 

出 版 物：《Journal of Behavioral and Brain Science》 (行为与脑科学期刊（英文）)

年 卷 期：2015年第5卷第7期

页      面：318-331页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Predator Odor 2 5-Dihydro-2 4 5-Trimethylthiazoline c-fos mRNA In-Situ-Hybridization Innate Fear Freezing GAD67 Mice 

摘      要：The synthetic predator odor 2,5-dihydro-2,4,5-trimethylthiazoline (TMT) was used to induce innate fear in male GAD67 mice and behavioral changes were correlated with c-fos mRNA levels as marker for neuronal activation to reveal underlying activated fear circuits. Results show the same amount of increased freezing and decreased rearing and grooming behavior of TMT-exposed GAD67 mice and wild type littermates, and therefore suggest that heterozygous knock-in of GFP in the GAD67 gene that is associated with a fifty percent decreased GAD67 protein level in the brain, has no impact on TMT-induced behavioral effects. Exposure to TMT significantly increased the number of c-fos mRNA positive cells in the main olfactory bulb (MOB), the lateral septum (LS), the bed nucleus of the stria terminalis (BNST), the central amygdala (CeA), the anterior-ventral (MeAav), the anterior-dorsal (MeAad) and the posterior-ventral (MeApv) part of the medial amygdala in GAD67 mice. Thus, to further investigate the role of GABAergic neurons in TMT-induced uncontrollable stress responses GAD67 mice that provide the advantage of prelabeled GABAergic neurons through the GABA neuron specific expression of GFP could be a suitable model organism.