Lignin reduction in transgenic poplars by expressing antisense CCoAOMT gene

作     者：LU Jing 1,3 *, ZHAO Huayan 1*, WEI Jianhua 2, HE Yikun 3, SHI Chao 1, WANG Hongzhi 2 and SONG Yanru 1**(1. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China 2. Beijing Center of Agro Biotechnology, Beijing Institute of Agriculture and Forestry, Beijing 100089, China 3. Department of Biology, Capital Normal University, Beijing 100037, China) 

作者机构：KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiologyInstituteofBotanyChineseAcademyofSciencesBeijing100093China／／DepartmentofBiologyCapitalNormalUniversityBeijing100037China KeyLaboratoryofPhotosynthesisandEnvironmentalMolecularPhysiologyInstituteofBotanyChineseAcademyofSciencesBeijing100093China BeijingCenterofAgro-BiotechnologyBeijingInstituteofAgricultureandForestryBeijing100089China DepartmentofBiologyCapitalNormalUniversityBeijing100037China 

出 版 物：《Progress in Natural Science:Materials International》 (自然科学进展·国际材料(英文))

年 卷 期：2004年第12期

页      面：28-31页

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：SupportedbytheNationalNaturalScienceFoundationofChina(GrantNo.30170783) NaturalScienceFoundationofBeijing(GrantNo.5012009)andtheState“863”HighTechProgram(GrantNo.2004AA212121) 

主　　题：Populus tomentosa CCoAOMT Agrobacterium tumefaciens transgene lignin. 

摘      要：The antisense Caffeoyl CoA O methyltransferase (CCoAOMT) cDNA was transformed into Chinese white poplar ( Populus tomentosa ) mediated by Agrobacterium tumefaciens . Many factors affecting the transformation efficiency were studied and a stable transformation system was established. PCR Southern blot analysis indicated that antisense CCoAOMT cDNA had been integrated into the genome of the transgenic poplars. RT PCR and Western blot analyses demonstrated that the endogenous CCoAOMT gene was suppressed at both transcriptional and translational levels. Klason lignin content assay exhibited the lignin reduction to different degrees in transgenic poplars. The stems of partial transgenic poplars with the remarkable lignin reduction turned red, and the color distribution was stripped or spotted. Taken together, these results suggested that CCoAOMT gene would be a potential useful gene in altering lignin biosynthesis by biotechnology for improving wood properties.