Comparison between different methods of estimation of vitamin D

作     者：Mrinal Pal Subinay Datta Amit Kumar Pradhan Lekha Biswas Joydeep Ghosh Partha Mondal Rajarshi Rahut Arghya Ray Chaudhuri Sourav Sau Soumi Das 

作者机构：Department of Biochemistry Burdwan Medical College Burdwan India Department of Radiotherapy Burdwan Medical College Burdwan India 

出 版 物：《Advances in Biological Chemistry》 (生物化学进展（英文）)

年 卷 期：2013年第3卷第5期

页      面：501-504页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Vitamin D High-Pressure Liquid Chromatography Chemiluminescent Immunoassays Enzyme Immunoassays 

摘      要：Background: Vitamin D is not only important for bone health but can also affect the development of several non-bone diseases. These findings have increased the need for determining vitamin D status in a convenient and cost-effective way. In order to determine the better method for detecting vitamin D status, we have estimated blood vitamin D in three methods such as high-pressure liquid chromatography, chemiluminescent immunoassays and enzyme immunoassays. Method: Two hundred and sixteen subjects irrespective of age and sex were studied for blood vitamin D in all the three methods over a period of 2 years. Result: The statistical analysis showed that values of vitamin D obtained by chemiluminescent immunoassays (r = 0.978,