Polymeric Nanohydrogels of Poly(N-Isopropylacrylamide)Combined with Others Functionalized Monomers:Synthesis and Characterization

作     者：Lissette Aguero Luztono Yuneivy Cepero Donates Luis Guillermo Guerrero Ramirez Addys Gonzalez Palomo Dionisio Zaldivar Silva Issa Katime 

作者机构：Departamento de Quimica MacromolecularCentro de BiomaterialesUniversidad de La HabanaHabanaCuba Grupo de Nuevos Materiales y Espectroscopia SupramolecularFacultad de Ciencias y TecnologiaBilbaoEspana Departamento de Biologia de SistemasCentro de Inmunologia MolecularHabanaCuba 

出 版 物：《Journal of Biomaterials and Nanobiotechnology》 (生物材料与纳米技术（英文）)

年 卷 期：2014年第5卷第1期

页      面：31-38页

学科分类：07[理学] 0703[理学-化学] 

基　　金：Financial support for this work from Ministerio de Ciencia y Tecnologia is gratefully acknowledged(MICINN) 

主　　题：N-Isopropylacrylamide Nanohydrogels Inverse Microemulsion Polymerization In Vitro Biocompatibility 

摘      要：Nanohydrogels from inverse microemulsion (w/o) polymerization, at 25°C, of N-isopropylacrylamide (NIPA) and functionalized monomers are described. The functionalized monomers were: N-(pyridine-4-ylmethyl) acrylamide (NP4MAM) and tert-butyl 2-acrylamidoethyl carbamate (2AAECM). The polymeric nanohydrogel obtained was characterized by attenuated total reflectance Fourier-transformed infrared spectroscopy (ATR-FTIR) and proton nuclear magnetic resonance spectrometry (1HNMR), while their morphology and particle size was assessed by scanning electron microscopy (SEM) and dynamic light scattering. Their thermal properties were studied by Differential Scanning Calorimetry (DSC) and Thermogravimetric Analysis (TGA). As a preliminary measure of biocompatibility, in vitro evaluations of the nanohydrogels were carried out by cellular toxicity (colon carcinoma cells, CT-26) and hemocompatibility tests. These evaluations showed that these nanohydrogels were not toxic in the examined concentration range and exhibited preliminary blood compatibility;therefore they could be used in biomedical applications.