Isolation and Identification of Two Antibacterial Agents from <i>Chromolaena odorata</i>L. Active against Four Diarrheal Strains
Isolation and Identification of Two Antibacterial Agents from <i>Chromolaena odorata</i>L. Active against Four Diarrheal Strains作者机构：CNRS UMR7178 University of Strasbourg Strasbourg France EA 7290 University of Strasbourg Strasbourg France Faculté de Chirurgie Dentaire Université de Strasbourg Strasbourg France Inserm UMR 1121 University of Strasbourg Strasbourg France IPHC-DSA CNRS University of Strasbourg Strasbourg France Université d’Abomey-Calavi Laboratoire de Biochimie et Biologie Moléculaire (LBBM) Cotonou Bénin
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2013年第3卷第1期
页 面：115-121页
学科分类：1006[医学-中西医结合] 1005[医学-中医学] 10[医学]
主 题：Chromolaena odorata 3’ 4’ 5 6 7-Pentamethoxyflavone (Sinensetin) 7-Tetramethoxyflavone (Scutellareintetramethyl ether) Antibacterial Activity Diarrheal Strains
摘 要：Chromolaena odorata L (Asteraceae) is a bad invasive plant, found in the humid tropics and sub-tropics worldwide. It is used against dysentery, diarrhea, malaria, wound healing, headache and toothache in traditional medicine. In the present study, we investigated the antibacterial activities of different leaves extracts of Chromolaena odorata L. (cyclohexane, dichloromethane, ethyl acetate and butanol) against four clinical diarrheal strains (Klebsiella oxytoca, Salmonella enterica, Shigella sonnei and Vibrio cholera). We demonstrated that C. odorata leaves extracts show an antibacterial activity between 0.156 and 1.25 mg/mL. Bioassay-guided chromatography by bioautography with iodonitrotetrazolium-based colorimetric assay allowed the isolation and identification of two active compounds. After the combination of RP-HPLC, mass spectrometry analysis, 1D and 2D-NMR spectroscopy, we isolated and characterized two active molecules corresponding to 3’,4’,5,6,7-Pentamethoxyflavone (Sinensetin) and4’,5,6,7-Tetramethoxyflavone (Scutellareintetramethyl ether).
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