Investigation of an inflammatory viral disease HBV in cardiac patients through polymerase chain reaction

作     者：Farina Saher Khalil ur Rehman Javed A.Qureshi Muhammad Irshad Hafiz M.Nasir Iqbal 

作者机构：Department of Chemistry and BiochemistryUniversity of AgricultureFaisalabadPakistan Health Biotechnology DivisionNational Institute for Biotechnology and Genetic Engineering(NIBGE)FaisalabadPakistan Department of MicrobiologyUniversity of AgricultureFaisalabadPakistan 

出 版 物：《Advances in Bioscience and Biotechnology》 (生命科学与技术进展（英文）)

年 卷 期：2012年第3卷第4期

页      面：417-422页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：HBV Liver Inflammation LFTs PCR Agarose Gel Electrophoresis 

摘      要：In the present study 100 cardiac patients were randomly selected from the cardiology ward, Allied Hospital Faisalabad, Pakistan. All the selected cases were analysed for different parameters like Hepatitis B surface Antigen (HbsAg), Bilirubin, Alkaline phosphatase, serum glutamic pyruvic transaminase, and serum glutamic-oxaloacetic transaminase. Out of total 16% patients were lying in the age of 21-30 year, 25% in the age of 31-40 year, 35% in the age of 41-50 year, 19% in the age of 51-60 years and 5% patients in the age of 61-70 years. No subject was found positive in age group 21-30 years patients. 35% patients have higher value of SGPT while, other 26% were with higher value of SGOT. Rest of the 32 and 24% have higher ALP and Bilirubin levels, respectively. Assay profile revealed that ALP level was increased with increasing age, body mass index, Creactive protein, diabetes, smoking, sex, serum uric acid, lead, cadmium, hypercholesterolemia, lesion of liver and cardiovascular disease. The serum of the eight HbsAg positive cases were tested for the presence of HBV through PCR and no sample was found positive. At the end of the study, PCR amplified samples were run on 1.5% agarose gel to confirm the case.